Discriminative Determination of Copper, Iron and Magnesium in Chlorophyll and Chlorophyllin Complexes and Their Contents in Foods
Discriminative Determination of Copper, Iron and Magnesium in Chlorophyll and Chlorophyllin Complexes and Their Contents in Foods
复制标题
食品中叶绿素及叶绿素复合物中铜、铁、镁及其含量的判别测定
DOI：
10.3358/shokueishi.34.25
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
M. Aoyama
中科院分区：
文献类型：
--
作者：
Koujun Tsunoda;N. Inoue;Mitsuo Tachibana;M. Aoyama
建立了一种简便的测定叶绿素和叶绿素络合物中铜、铁、镁的方法。蔬菜样品用乙醇提取，口香糖样品用乙酸正丁酯、乙醇和水提取。利用醋酸丁酯-乙醇-水(10：40：60)的分配层析，在pH 8~9或pH 5~6的条件下，对提取物中的金属叶绿素(铜、铁、镁)和金属叶绿素(铜、铁、镁)进行了纯化和分离。用火焰原子吸收光谱仪测定了被鉴别的叶绿素和叶绿素中的金属含量。金属叶绿素和叶绿素的回收率分别为：铜，88%，铁，%，镁，79%，铜，87%，铁，80%，镁，87%。我们对36种蔬菜和5种口香糖中的金属叶绿素和叶绿素进行了测定，发现有些含有0.1ppm以上的金属叶绿素和叶绿素。
A convenient discriminative determination of copper (Cu), iron (Fe), magnesium (Mg) in chlorophyll and chlorophyllin complexes was developed. Vegetable samples were extracted with ethanol, and chewing gum samples with n-butyl acetate, ethanol and water. The metal chlorophyll (Cu, Fe and Mg) and metal chlorophyllin (Cu, Fe and Mg) in the extracts were purified and separated at either pH 8-9 or pH 5-6 by making use of partition chromatography with n-butyl acetate-ethanol-water (10:40:60). The metal in the discriminated chlorophyll and chlorophyllin was determined with a flame-type atomic absorption spectrometer.The recoveries of metal chlorophylls and chlorophyllins were as follows: Cu chlorophyll, 88%; Fe chlorophyll, 64%; Mg chlorophyll, 79% and Cu chlorophyllin, 87%; Fe chlorophyllin, 80%; Mg chlorophyllin, 87%.We have carried out a survey on metal chlorophyll and chlorophyllin in 36 vegetables and 5 chewing gums and found that some of them contained metal chlorophylls and chlorophyllins at the level of 0.1ppm or over.