Regenerative Effects of Basic Fibroblast Growth Factor on Restoration of Thyroarytenoid Muscle Atrophy Caused by Recurrent Laryngeal Nerve Transection
Regenerative Effects of Basic Fibroblast Growth Factor on Restoration of Thyroarytenoid Muscle Atrophy Caused by Recurrent Laryngeal Nerve Transection
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DOI：
10.1016/j.jvoice.2017.09.019
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发表时间：
2018-11-01
期刊：
影响因子：
2.2
通讯作者：
Hirano, Shigeru
中科院分区：
文献类型：
--
作者：
Kaneko, Mami;Tsuji, Takuya;Hirano, Shigeru
目标.单侧声带麻痹后的声带萎缩是由甲杓肌萎缩引起的，仍然是一个挑战。内侧化手术是流行的治疗选择;然而，由于TA肌肉的质量或张力减少，声音嘶哑通常仍然存在。因此，除了内侧化手术，TA肌肉神经再支配是可取的。体内研究表明，碱性成纤维细胞生长因子（bFGF）有可能影响肌肉和神经再生。本研究旨在研究bFGF对喉返神经切断引起的TA肌萎缩的再生作用。前瞻性动物实验与对照。TA肌萎缩是由单侧喉返神经切断引起的。切断后1个月，在50 μ L中重复注射不同剂量（200 ng，100 ng，10 ng）的bFGF到TA肌肉中，注射间隔1周，共4次。假手术组只注射生理盐水。最后一次注射后4周取喉进行组织学和免疫组化检查。与假手术组相比，bFGF治疗组的横截面TA肌肉面积显著更大。免疫组化显示bFGF显著增加TA肌中神经肌肉接头和卫星细胞的数量。结果提示，局部应用bFGF可改善喉返神经麻痹引起的TA肌萎缩。此外，bFGF可能对神经和肌肉都有再生作用。
Objectives. Vocal fold atrophy following unilateral vocal fold paralysis is caused by atrophy of the thyroarytenoid (TA) muscle and remains a challenge. Medialization procedures are popular treatment options; however, hoarseness often remains due to the reduction in mass or tension of the TA muscle. Therefore, in addition to medialization procedures, TA muscle reinnervation is desirable. In vivo studies have shown the potential for basic fibroblast growth factor (bFGF) to affect muscular and nerve regeneration. The present study aimed to examine the regenerative effects of bFGF on restoration of TA muscle atrophy caused by recurrent laryngeal nerve transection.Study design. Prospective animal experiments with controls.Methods. TA muscle atrophy was induced by unilateral transection of the recurrent laryngeal nerve. One month after transection, different doses (200 ng, 100 ng, 10 ng) of bFGF in 50 mu L were repeatedly injected into the TA muscle four times with an interval of 1 week between injections. Saline only was injected in the sham group. Larynges were harvested for histologic and immunohistochemical examination 4 weeks after the final injection.Results. The cross-sectional TA muscle area was significantly larger in the bFGF-treated groups compared with the sham-treated groups. Immunohistochemistry indicated that bFGF significantly increases the number of neuromuscular junctions and satellite cells in the TA muscle.Conclusions. These results suggest that local application of bFGF to the TA muscle may improve TA muscle atrophy caused by recurrent laryngeal nerve paralysis. Furthermore, bFGF may have regenerative effects on both nerves and muscles.