Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park
Thermocrinis ruber gen. nov., sp. nov., a Pink-Filament-Forming Hyperthermophilic Bacterium Isolated from Yellowstone National Park
复制标题
DOI：
10.1128/aem.64.10.3576-3583.1998
复制
发表时间：
1998-10
影响因子：
4.4
通讯作者：
R. Huber;W. Eder;Stefan Heldwein;G. Wanner;H. Huber;R. Rachel;K. Stetter
中科院分区：
文献类型：
--
作者：
R. Huber;W. Eder;Stefan Heldwein;G. Wanner;H. Huber;R. Rachel;K. Stetter
摘要从美国怀俄明州黄石国家公园章鱼泉上游流出通道（温度82 - 88°C）的粉红色丝状流（粉红色细丝）中分离到一种新的超嗜热细菌。革兰氏阴性细胞生长在低盐度和高达89°C的温度下，在中性至碱性pH范围内。取决于培养条件，生物体以单个能动杆、聚集体或形成流光状细胞团的长丝形式出现。新的分离物生长chemolithoautotrophically与氢，硫代硫酸盐，元素硫作为电子供体和氧作为电子受体。或者，在有氧条件下，甲酸盐和甲酰胺作为唯一的能量和碳源。新分离物的16 S rRNA序列与从未培养的粉红色花丝中获得的16 S rRNA序列密切相关。它是水生目中的一个新属，我们将其模式种命名为红色热滨藻（Thermocrinis ruber）（模式株，OC 1/4 [= DSM 12173]）。
ABSTRACT A novel hyperthermophilic bacterium was isolated from pink filamentous streamers (pink filaments) occurring in the upper outflow channel (temperature, 82 to 88°C) of Octopus Spring in Yellowstone National Park, Wyo. The gram-negative cells grew at low salinity at temperatures up to 89°C in the neutral to alkaline pH range. Depending on the culture conditions, the organisms occurred as single motile rods, as aggregates, or as long filaments that formed streamer-like cell masses. The novel isolate grew chemolithoautotrophically with hydrogen, thiosulfate, and elemental sulfur as electron donors and oxygen as the electron acceptor. Alternatively, under aerobic conditions, formate and formamide served as sole energy and carbon sources. The novel isolate had a 16S rRNA sequence closely related to the 16S rRNA sequence obtained from uncultivated pink filaments. It represents a new genus in the orderAquificales, the type species of which we nameThermocrinis ruber (type strain, OC 1/4 [= DSM 12173]).