Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity
Cholesterol lowering in pigs through enhanced bacterial bile salt hydrolase activity
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DOI：
10.1079/bjn19980030
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发表时间：
1998-02-01
影响因子：
3.6
通讯作者：
Verstraete, W
中科院分区：
文献类型：
--
作者：
De Smet, I;De Boever, P;Verstraete, W
本文研究了饲喂含有活性胆盐水解酶（BSH）的罗伊氏乳杆菌活细胞对猪血浆胆固醇水平的影响。在为期13周的实验中，20头猪在前10周喂食高脂肪，高胆固醇，低纤维饮食，最后3周喂食普通猪饮食。一组动物每天两次接受11.25（SD 0.16）log（10）菌落形成单位的潜在益生菌，持续4周（从第3周至第7周）。从第8周开始，给药组再次饲喂与对照组相同的饲料，不添加任何添加剂。粪便中乳酸杆菌总数仅在前2周内显著高于处理组。reuteri喂养。基于有限的数据，有人提出，所施用的乳酸杆菌属物种引起了土著乳酸杆菌种群的暂时转移，而不是永久地定殖在肠道中。与对照猪相比，益生菌饲喂显著降低了处理猪的总胆固醇和LDL-胆固醇浓度（P <或等于0.05），而没有观察到HDL-胆固醇浓度的变化。中性固醇和胆汁盐的粪便排出量数据在各组动物之间差异很大，但它们表明给药猪的排出量增加。虽然在乳酸杆菌给药期后的3周内，两组的血液胆固醇水平均升高，但在给药猪中观察到血清总胆固醇和LDL-胆固醇水平显著降低。在正常饮食的最后3周期间，两个动物组的胆固醇浓度显著降低，组间总胆固醇和LDL-胆固醇浓度的差异基本消失。
The effect of feeding live Lactobacillus reuteri cells containing active bile salt hydrolase (BSH) on plasma cholesterol levels was studied in pigs. During an experiment lasting 13 weeks, twenty pigs were fed on a high-fat, high-cholesterol, low-fibre diet for the first 10 weeks, and a regular pig diet for the last 3 weeks. One group of animals received, twice daily, 11.25 (SD 0.16) log(10) colony forming units of the potential probiotic bacteria for 4 weeks (from week 3 until week 7). From week 8 onwards, the treated group was again fed on the same diet as the control group without additions. The total faecal Lactobacillus counts were only significantly higher in the treated pigs during the first 2 weeks of L. reuteri feeding. Based on limited data, it was suggested that the administered Lactobacillus species had caused a temporary shift within the indigenous Lactobacillus population rather than permanently colonizing the intestinal tract. The probiotic feeding brought about significant lowering (P less than or equal to 0.05) of total and LDL-cholesterol concentrations in the treated pigs compared with the control pigs, while no change in HDL-cholesterol concentration was observed. The data for faecal output of neutral sterols and bile salts were highly variable between the animals of each group, yet they indicated an increased output in the treated pigs. Although the blood cholesterol levels went up in both groups during the 3 weeks following the Lactobacillus administration period, significantly lower serum total and LDL-cholesterol levels were observed in the treated pigs. During the final 3 weeks of normalization to the regular diet, cholesterol concentrations significantly decreased in both animal groups and the differences in total and LDL-cholesterol concentrations between the groups largely disappeared.