Recombinant engineering of reversible cross-links into a resilient biopolymer
Recombinant engineering of reversible cross-links into a resilient biopolymer
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DOI：
10.1016/j.polymer.2015.03.030
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发表时间：
2015-07-09
期刊：
影响因子：
4.6
通讯作者：
Harrington, Matthew J.
中科院分区：
文献类型：
--
作者：
Degtyar, Elena;Mlynarczyk, Barbara;Harrington, Matthew J.
Metal coordination bonds are employed in protein based biological materials such as the mussel byssus as reversible sacrificial bonds to achieve autonomic and intrinsic self-healing behavior. In the present study, histidine residues capable of forming coordination bonds were recombinantly engineered into the consensus sequence of resilin, an insect protein that forms a rubbery and resilient biopolymeric network. The purified recombinant resilin mutant, AnG_2His16, was photo cross-linked to form thin films that exhibited a 30-fold increase in modulus compared with wild-type resilin sequences. Addition of Zn2+ ions to the mutant resilin films, led to a further increase in stiffness, which, according to Raman spectroscopic studies, arises from His-metal coordination cross-links. These findings show the potential for tuning mechanics and self-healing behavior in biopolymers using bio-engineered metal-binding sites. (C) 2015 Elsevier Ltd. All rights reserved.
