Synthesis and accumulation of protein and carbohydrases along the rat villus column.
Synthesis and accumulation of protein and carbohydrases along the rat villus column.
复制标题
蛋白质和糖酶沿着大鼠绒毛柱的合成和积累。
DOI：
10.1097/00005176-199110000-00001
复制
发表时间：
1991
影响因子：
2.9
通讯作者：
Tsuboi,KK
中科院分区：
文献类型：
--
作者：
Castillo,RO;Kwong,LK;Tsuboi,KK
摘要： 幼年和成年大鼠肠粘膜的肠细胞在隐窝中不断增殖，沿绒毛柱迁移而成熟，并从绒毛尖端排出。我们检查了总蛋白、乳糖酶-根皮苷水解酶、蔗糖酶-异麦芽糖酶和麦芽糖酶-葡糖淀粉酶的合成模式，以及这些酶在沿绒毛迁移过程中在细胞中的积累。将标记的亮氨酸腹膜内给予乳鼠和年轻成年大鼠，并测定从绒毛柱顶端到基部顺序分离的发育中绒毛细胞的蛋白质和消化糖酶池中的放射性。研究发现，发育中的细胞在沿着绒毛柱上升时不断积累蛋白质和糖酶。此外，沿着绒毛的长度，放射性掺入总蛋白和糖酶池中的速率通常是恒定的。这些研究表明，婴儿和年轻成年大鼠肠道的分化肠上皮细胞在迁移绒毛柱长度的同时表现出持续生长的模式，并在此期间以大致恒定的速率维持蛋白质和消化糖酶的合成。
Summary: Enterocytes of the intestinal mucosa of infant and adult rats continuously proliferate in the crypt, mature as they migrate along the villus column, and are discharged from the villus tip. We examined the synthesis patterns of total protein, lactase‐phlorizin hydrolase, sucrase‐isomaltase, and maltase‐glucoamylase as well as the accumulation of these enzymes in cells during migration along the villus. Labeled leucine was administered intraperitoneally to suckling and young adult rats, and radioactivity was determined in protein and digestive carbohydrase pools of developing villus cells separated sequentially from tip to base of the villus column. The developing cells were found to continuously accumulate protein and carbohydrases as they ascended the villus column. In addition, incorporation of radioactivity into total protein and carbohydrase pools occurred at generally constant rates along the length of the villus. These studies showed that the differentiated enterocyte of both infant and young adult rat intestine exhibits a pattern of continuous growth while migrating the length of the villus column and maintains synthesis of protein and digestive carbohydrases at generally constant rates during this time.