Cytokine Gene Polymorphisms Associated With Rheumatoid Arthritis and Periodontitis in Japanese Adults
Cytokine Gene Polymorphisms Associated With Rheumatoid Arthritis and Periodontitis in Japanese Adults
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DOI：
10.1902/jop.2009.080573
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发表时间：
2009-05-01
影响因子：
4.3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
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作者：
Kobayashi, Tetsuo;Murasawa, Akira;Yoshie, Hiromasa
背景：细胞因子在类风湿关节炎（RA）和牙周炎的发病机制中起重要作用。这两种疾病以前被证明是部分受细胞因子基因多态性的影响。因此，我们评估是否是唯一的受试者与两种diseases.Methods的细胞因子基因型的分布：研究对象包括日本成人RA（RA组; n = 153），牙周炎（P组; n = 117），和健康人（H组; n = 108）。临床牙周状况是通过测量探诊深度、临床附着水平和探诊出血来确定的。从外周血中提取基因组DNA，检测白细胞介素（IL）-1、-2、-4、-6、-10、肿瘤坏死因子α（TNF-α）和转化生长因子β 1（TGF-β 1）等16个基因多态性。结果：RA + P组137例，牙周炎发生率为89.5%。在受试者组中，未观察到任何细胞因子基因型和等位基因频率的显著差异。在调整年龄、性别和吸烟状况后，多因素Logistic回归分析显示RA + P组和P组IL-1B +3954基因型分布差异有统计学意义RA + P组与H组比较P=0.006（P = 0.008）。患有RA和牙周炎的日本个体可能表现出不同的IL-IB +分布3954个基因型，比健康对照组和仅患牙周炎的受试者高。牙周病学杂志2009;80：792-799。
Background: Cytokines play a major role in the pathogenesis of rheumatoid arthritis (RA) and periodontitis. Both diseases were previously shown to be partly influenced by cytokine gene polymorphisms. Therefore, we evaluated whether the distributions of the cytokine genotypes were unique to subjects with both diseases.Methods: The study subjects consisted of Japanese adults with RA (RA group; n = 153), periodontitis only (P group; n = 117), and healthy individuals (H group; n = 108). Clinical periodontal condition was defined by measurements of probing depth, clinical attachment level, and bleeding on probing. Genomic DNA was isolated from peripheral blood and analyzed for the determination of 16 gene polymorphisms encoding interleukin (IL)-1, -2, -4, -6, and -10, tumor necrosis factor-alpha, and transforming growth factor-beta 1.Results: The frequency of patients with RA who exhibited periodontitis was 89.5% (RA + P group; n = 137). No significant differences were observed in any of the frequencies of cytokine genotypes and alleles among the subject groups. After adjustment for age, gender, and smoking status, multiple logistic regression analysis revealed a significant difference in the distribution of IL-IB +3954 genotypes between RA + P and P groups (P=0.006) and between RA + P and H groups (P = 0.008).Conclusion: Japanese individuals with RA and periodontitis may exhibit different distributions of IL-IB +3954 genotypes than healthy controls and subjects with periodontitis only. J Periodontol 2009;80:792-799.