A transformation-competent recombinant between v-src and Rous-associated virus RAV-1.
A transformation-competent recombinant between v-src and Rous-associated virus RAV-1.
复制标题
v-src 和 Rous 相关病毒 RAV-1 之间的具有转化能力的重组体。
DOI：
10.1128/jvi.64.4.1873-1877.1990
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发表时间：
1990
影响因子：
5.4
通讯作者：
Guntaka,RV
中科院分区：
文献类型：
--
作者：
Svoboda,J;Kandala,JC;Geryk,J;Pichrtova,J;Guntaka,RV
LTR、v-src、LTR原病毒是由H-19仓鼠肿瘤中src mRNA的逆转录和整合而产生的，通过与感染劳斯相关病毒RAV-1的鸡成纤维细胞融合成功地恢复了这些原病毒。一个获救的病毒E6获得gag基因结构5'端的1千碱基。重组发生在v-src外显子（位置7054）和gag（位置1417）之间的15个核苷酸同源区域。这种重组导致src剪接受体位点序列的改变，但该位点作为一个功能性剪接受体位点被维持。重组E6病毒长末端重复序列的核苷酸结构表明，它是由反转录从RAV-1跳到src mRNA的分子间跳跃引起的，然后在已经描述的同源区域之间切换模板。反转录的第二次跳跃显然是一个分子内事件。E6获得了1千碱基的5' gag，维持了未剪接和剪接的E6 rna的平衡，并确保了获救的E6病毒比获救的F6病毒具有复制优势，后者的基因组与祖先H-19细胞中的基因组相对应。
The LTR, v-src, LTR provirus, which arose by the reverse transcription and integration of src mRNA in the H-19 hamster tumor, has been successfully rescued by fusion with chicken fibroblasts infected with Rous-associated virus RAV-1. One rescued virus, E6, acquired 1 kilobase of the 5' end of the gag gene structure. Recombination took place in the region of 15-nucleotide homology exactly between v-src exon (position 7054) and gag (position 1417). This recombination resulted in the alteration of src splice acceptor site sequences, but this site is maintained as a functional splice acceptor site. The nucleotide structure of the long terminal repeat of recombinant E6 virus suggests that it arose by the intermolecular jump of reverse transcription from RAV-1 to src mRNA and then the switch of templates between already depicted regions of homology. The second jump of reverse transcription was apparently an intramolecular event. The acquisition of 1 kilobase of the 5' gag by E6 resulted in maintaining the balance of unspliced and spliced E6 RNAs and assured the replication advantage of rescued E6 virus over rescued F6 virus, the genome of which corresponds to that present in ancestral H-19 cells.