Bionomics and insecticide resistance of Aedes albopictus in Shandong, a high latitude and high-risk dengue transmission area in China
Bionomics and insecticide resistance of Aedes albopictus in Shandong, a high latitude and high-risk dengue transmission area in China
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中国高纬度登革热高发区山东省白纹伊蚊的生物学特征及抗药性
DOI：
10.1186/s13071-020-3880-2
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发表时间：
2020-01-09
影响因子：
3.2
通讯作者：
Gong, Maoqing
中科院分区：
文献类型：
--
作者：
Liu, Hongmei;Liu, Luhong;Gong, Maoqing
研究背景我国登革热疫情有向北蔓延的趋势，济宁是目前发现本地登革热病例的最北地区。因此，研究白纹伊蚊的密度及其对溴氰菊酯的抗性具有重要意义。方法通过计算Breteau指数（BI）和容器指数（CI）来评估白纹伊蚊幼虫密度。 2017年和2018年在济宁市任城、兖州、泗水、梁山、邹城、嘉祥6个县开展白纹伊蚊和人饵双网诱捕监测。使用世界卫生组织 (WHO) 标准耐药性生物测定法评估白纹伊蚊成虫对溴氰菊酯的耐药性。还分析了Vgsccodon 1532和1534的突变，以确定kdr突变与成年蚊子表型抗性之间的关联。结果济宁市2017年的平均BI、CI和叮咬率分别为45.30、16.02和1.97（雌性/人/小时），2017年为15.95、7.86和0.59 f/m/h分别于2018年。 2017年8月26日，确诊首例登革热病例时，嘉祥县千里村BI为107.27。政府采取的防控措施使 2017 年 9 月 3 日 BI 值大幅下降至 4.95。嘉祥地区白纹伊蚊为41.98%。在Vgsc基因的结构域III中发现了I1532T、F1534L和F1534S突变。本研究首次证明I1532T和F1534S突变与溴氰菊酯耐药表型呈正相关。结论应加强登革热高危地区蚊子密度监测、耐药性监测和风险评估，确保伊蚊的可持续控制。白纹伊蚊，从而预防和控制登革热传播。
BackgroundDengue fever outbreaks tend to spread northward in China, and Jining is the northernmost region where local dengue fever cases have been detected. Therefore, it is important to investigate the density ofAedes albopictusand its resistance to deltamethrin.MethodsThe Breteau index (BI) and container index (CI) were calculated to assess the larval density ofAe. albopictusand human-baited double net trap (HDN) surveillance was performed in six subordinate counties (Rencheng, Yanzhou, Sishui, Liangshan, Zoucheng and Jiaxiang) of Jining City in 2017 and 2018. The resistance ofAe. albopictusadults to deltamethrin was evaluated using the World Health Organization (WHO) standard resistance bioassay. The mutations atVgsccodons 1532 and 1534 were also analysed to determine the association betweenkdrmutations and phenotypic resistance in adult mosquitoes.ResultsThe average BI, CI and biting rate at Jining were 45.30, 16.02 and 1.97 (female /man/hour) in 2017 and 15.95, 7.86 and 0.59 f/m/h in 2018, respectively. In August 26, 2017, when the first dengue fever case was diagnosed, the BI at Qianli village in Jiaxiang County was 107.27. The application of prevention and control measures by the government sharply decreased the BI to a value of 4.95 in September 3, 2017. The mortality of field-collectedAe. albopictusfemales from Jiaxiang was 41.98%. I1532T, F1534L and F1534S mutations were found in domain III of theVgscgene. This study provides the first demonstration that both I1532T and F1534S mutations are positively correlated with the deltamethrin-resistant phenotype.ConclusionsMosquito density surveillance, resistance monitoring and risk assessment should be strengthened in areas at risk for dengue to ensure the sustainable control ofAe. albopictusand thus the prevention and control of dengue transmission.