Inhibition of Lupus Anticoagulant Activity by Hexagonal Phase Phosphatidylethanolamine in the Presence of Prothrombin
Inhibition of Lupus Anticoagulant Activity by Hexagonal Phase Phosphatidylethanolamine in the Presence of Prothrombin
复制标题
凝血酶原存在下六角相磷脂酰乙醇胺对狼疮抗凝活性的抑制
作者：
J. Rauch;M. Tannenbaum;C. Neville;P. Fortin
我们先前已经证明，来自系统性红斑狼疮（SLE）患者的狼疮抗凝抗体特异性识别六方（II）相磷脂酰乙醇胺（PE），但不识别双层PE（Thromb Haemost 1989; 62：892）。在这些研究中，没有评估蛋白质参与这种识别。为了解决这个问题，我们已经从SLE患者的血浆中分离出IgG狼疮抗凝抗体，并评估了在存在和不存在纯化的血浆蛋白的情况下六边形（II）相PE对狼疮抗凝活性的抑制。所有六个IgG狼疮抗凝抗体测试抑制六边形（II）相PE的存在下，但不是不存在，人凝血酶原。相比之下，单独使用凝血酶原或PE与β2-糖蛋白I或膜联蛋白V的组合几乎没有或没有观察到抑制。这些数据表明，对于某些狼疮抗凝抗体，六边形（II）相PE的抑制依赖于凝血酶原，表明这些抗体识别PE和凝血酶原的复合物。
Summary We have previously demonstrated that lupus anticoagulant antibodies from patients with systemic lupus erythematosus (SLE) specifically recognize hexagonal (II) phase phosphatidylethanolamine (PE), but not bilayer PE (Thromb Haemost 1989; 62: 892). In those studies, the involvement of proteins in this recognition was not evaluated. To address this issue, we have isolated IgG lupus anticoagulant antibodies from the plasma of SLE patients and evaluated the inhibition of lupus anticoagulant activity by hexagonal (II) phase PE in the presence and absence of purified plasma proteins. All six of the IgG lupus anticoagulant antibodies tested were inhibited by hexagonal (II) phase PE in the presence, but not the absence, of human prothrombin. In contrast, little or no inhibition was observed with prothrombin alone or with PE in combination with either β2-glycoprotein I or annexin V. These data indicate that, for certain lupus anticoagulant antibodies, inhibition by hexagonal (II) phase PE is dependent on prothrombin, suggesting that these antibodies recognize a complex of PE and prothrombin.
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DOI：
10.1016/s0021-9258(17)42183-1
发表时间：
1994-01
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Smirnov;C. Esmon
通讯作者：
M. Smirnov;C. Esmon
影响因子：
20.3
作者：
Rebecca A. Fleck;S. Rapaport;Vijaya Mohan Rao
通讯作者：
Rebecca A. Fleck;S. Rapaport;Vijaya Mohan Rao
影响因子：
20.3
作者：
K. Mann;M. Nesheim;WR Church;P. Haley;S. Krishnaswamy
通讯作者：
K. Mann;M. Nesheim;WR Church;P. Haley;S. Krishnaswamy
狼疮抗凝物 IgG 和凝血酶原与表面磷脂结合的机制和作用。
影响因子：
20.3
作者：
Rao,LV;Hoang,AD;Rapaport,SI
通讯作者：
Rapaport,SI