Deltabaculoviruses encode a functional type I budded virus envelope fusion protein
Deltabaculoviruses encode a functional type I budded virus envelope fusion protein
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Delta杆状病毒编码功能性I型芽状病毒包膜融合蛋白
DOI：
10.1099/jgv.0.000745
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发表时间：
2017
影响因子：
3.8
通讯作者：
Vlak Just M.
中科院分区：
文献类型：
--
作者：
Wang Manli;Shen Shu;Wang Hualin;Hu Zhihong;Becnel James;Vlak Just M.
包膜融合蛋白（F蛋白）是α-和β-杆状病毒（杆状病毒科）的芽殖病毒（BV）的主要成分，并且对于昆虫幼虫的系统感染和昆虫细胞培养是必需的。Anfhomologue基因在γ-杆状病毒中缺失。本文研究了黑须库蚊核型多角体病毒（Culex nigripalpus nucleopolyhedrovirus，CuniNPV）的F同源基因Cuni-F。当单独表达时，该蛋白似乎位于细胞表面，并且能够诱导细胞-细胞融合。当由α杆状病毒（苜蓿银纹夜蛾核型多角体病毒）表达时，发现其被并入BV中。Western blot分析检测到未切割的Cuni-F0和弗林蛋白酶切割的F1形式。用衣霉素处理感染的细胞表明Cuni-F含有N-聚糖。突变分析鉴定了典型的弗林蛋白酶切割位点126 RARR 129负责昆虫细胞中Cuni-F的切割。这些证据表明CuniNPV编码一种功能性F蛋白。
Envelope fusion proteins (F proteins) are major constituents of budded viruses (BVs) of alpha- and betabaculoviruses (Baculoviridae) and are essential for the systemic infection of insect larvae and insect cell culture. Anfhomologue gene is absent in gammabaculoviruses. Here we characterized the putative F-homologue (Cuni-F), encoded by (ORF) 104 ofCulex nigripalpusnucleopolyhedrovirus (CuniNPV), the only deltabaculovirus member. When expressed alone, this protein seems to locate on the cell surface and is able to induce cell–cell fusion. When expressed by an alphabaculovirus (Autographa californianucleopolyhedrovirus), it was found to be incorporated into BVs. Western blot analyses detected the uncleaved Cuni-F0and the furin-cleaved F1forms. Treatment of infected cells with tunicamycin showed that Cuni-F contains N-glycans. Mutagenesis analysis identified the canonical furin cleavage site126RARR129as being responsible for the cleavage of Cuni-F in insect cells. The collective evidence suggests that CuniNPV encodes a functional F protein.