Modulation of macrophage activity by aflatoxins B1 and B2 and their metabolites aflatoxins M1 and M2
Modulation of macrophage activity by aflatoxins B1 and B2 and their metabolites aflatoxins M1 and M2
复制标题
DOI：
10.1016/j.toxicon.2012.02.010
复制
发表时间：
2012-05-01
期刊：
影响因子：
2.8
通讯作者：
Severino, L.
中科院分区：
文献类型：
--
作者：
Bianco, G.;Russo, R.;Severino, L.
黄曲霉毒素是常见于食品和饲料中的天然污染物。其中许多在哺乳动物中发挥免疫调节作用;因此，本研究的目的是研究AFB 1，AFB 2，AFM 1和AFM 2单独和不同组合在J774A.1小鼠巨噬细胞中的免疫作用。MTT法显示，黄曲霉毒素B_1单独或与黄曲霉毒素B_2联合使用，只有在最高浓度时才具有抗增殖活性，其羟基化代谢产物没有这种作用。AFM 1和AFM 2。然而，在目前的研究中评估的黄曲霉毒素的免疫毒性作用可能是由于抑制生产的活性氧代谢产物，如NO。在巨噬细胞中暴露于黄曲霉毒素（10-100 μ M）的细胞荧光测定显示，它们的细胞毒性是不相关的凋亡途径。结果表明，黄曲霉毒素可影响巨噬细胞的功能，特别是AFB 1、AFB 2、AFM 1和AFM 2联合作用可产生相互作用，影响巨噬细胞的免疫反应性。(C)2012爱思唯尔有限公司保留所有权利。
Aflatoxins are natural contaminants frequently found both in food and feed. Many of them exert immunomodulatory properties in mammals; therefore, the aim of the current study was to investigate immune-effects of AFB1, AFB2, AFM1 and AFM2, alone and differently combined, in J774A.1 murine macrophages. MTT assay showed that AFB1, alone and combined with AFB2, possess antiproliferative activity only at the highest concentration; such effect was not shown by their hydroxylated metabolites. AFM1 and AFM2, respectively. However, the immunotoxic effects of the aflatoxins evaluated in the current study may be due to the inhibition of production of active oxygen metabolites such as NO. Cytofluorimetric assay in macrophages exposed to aflatoxins (10-100 mu M) revealed that their cytoxicity is not related to apoptotic pathways. Nevertheless, a significant increase of the 5 phase cell population accompanied by a decrease in G0/G1 phase cell population was observed after AFB1 treatment.In conclusion, the results of the current study suggest that aflatoxins could compromise the macrophages functions; in particular, co-exposure to AFB1, AFB2, AFM1 and AFM2 may exert interactions which can significantly affect immunoreactivity. (C) 2012 Elsevier Ltd. All rights reserved.