EXPRESSION OF C-KIT AND KIT-LIGAND PROTEINS IN NORMAL HUMAN TISSUES
EXPRESSION OF C-KIT AND KIT-LIGAND PROTEINS IN NORMAL HUMAN TISSUES
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DOI：
10.1177/42.11.7523489
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发表时间：
1994-11-01
影响因子：
3.2
通讯作者：
CORDONCARDO, C
中科院分区：
文献类型：
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作者：
LAMMIE, A;DROBNJAK, M;CORDONCARDO, C
C-kit受体及其同源配体KL在黑素合成、配子发生和造血过程中发挥重要作用。关于c-kit和KL表达的研究主要集中在小鼠的发育过程中。我们开展了这项研究，以确定这些分子在正常成人组织中的表达模式。利用免疫组织化学和同一块组织的连续切片，我们评估了各种保存良好的正常组织中c-kit和KL的显微解剖分布。C-KIT蛋白表达于组织肥大细胞、黑素细胞、乳腺、腮腺、真皮汗液和食管腺上皮细胞。睾丸和卵巢间质细胞也观察到散在的c-kit免疫反应。C-kit在中枢神经系统中有明显的区域分布，特别是在小脑、海马体和脊髓背角。KL蛋白存在于与受体染色互补的细胞中，如乳腺和汗腺的腺肌上皮细胞。在膀胱、宫颈、子宫和胃肠道的平滑肌细胞以及横纹肌和心肌中观察到强烈的KL免疫反应。KL在前列腺纤维肌间质细胞中也有较强的染色。在中枢神经系统中，KL的表达仅限于小脑的高尔基体和浦肯野细胞。这些结果表明，这种受体及其配体在维持各种完全分化的组织中发挥了作用。
The c-kit receptor and its cognate ligand, KL, play a critical role in melanogenesis, gametogenesis, and hematopoiesis. Studies on the expression of c-kit and KL have been primarily focused on mouse development. We undertook the present study to characterize the pattern of expression of these molecules in normal adult human tissues. Using immunohistochemistry and consecutive tissue sections from the same block, we evaluated a variety of well-preserved normal tissues for c-kit and KL microanatomic distribution. c-kit protein was identified in tissue mast cells, melanocytes, glandular epithelial cells of breast, parotid, dermal sweat, and esophageal glands. Scattered c-kit immunoreactivity was also observed for testicular and ovarian interstitial cells. A striking regional distribution of c-kit was detected in the central nervous system, particularly in the cerebellum, hippocampus, and dorsal horn of the spinal cord. KL protein was identified in cells complementary to staining for the receptor, such as glandular myoepithelium of breast and sweat glands. Intense KL immunoreactivity was observed in smooth muscle cells of the bladder, cervix, uterus, and gastrointestinal tract, as well as in striated and cardiac muscle. Strong KL staining was also detected in prostate fibromuscular stroma cells. In the central nervous system, KL expression was confined to Golgi and Purkinje cells in the cerebellum. These results suggest a role for this receptor and its ligand in the maintenance of a variety of fully differentiated tissues.