Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis
Transcriptional analysis of inducible acetamidase gene of Mycobacterium smegmatis
复制标题
DOI：
10.1016/s0378-1097(00)00447-x
复制
发表时间：
2000-11-15
影响因子：
2.1
通讯作者：
Narayanan, PR
中科院分区：
文献类型：
--
作者：
Narayanan, S;Selvakumar, S;Narayanan, PR
分离和分析分枝杆菌强的和可调控的启动子，将有助于帮助克隆基因在分枝杆菌中表达。本研究定位了耻垢分枝杆菌诱导型乙酰胺酶基因的转录起始位点，并对其调控机制进行了研究。北方印迹、逆转录-PCR和引物延伸分析研究用于确定启动子的位置。(C)2000年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Isolation and analysis of strong and regulatable promoters of mycobacteria should be useful tools to aid the expression of cloned genes in mycobacteria. In the present study, we have mapped the transcriptional start site of the inducible acetamidase gene of Mycobacterium smegmatis and studied the mechanism of its regulation. Northern blot, reverse transcription-PCR and primer extension analysis studies were used to determine the position of the promoter. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.