CD154/CD40 and CD70/CD27 interactions have different and sequential functions in T cell-dependent B cell responses: enhancement of plasma cell differentiation by CD27 signaling.
CD154/CD40 and CD70/CD27 interactions have different and sequential functions in T cell-dependent B cell responses: enhancement of plasma cell differentiation by CD27 signaling.
复制标题
DOI：
10.4049/jimmunol.159.6.2652
复制
发表时间：
1997-09
影响因子：
4.4
通讯作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
中科院分区：
文献类型：
--
作者：
S. Jacquot;T. Kobata;S. Iwata;C. Morimoto;S. Schlossman
CD 40是TNF受体家族成员，在T细胞介导的B细胞活化中起核心作用。我们最近证实，另一个TNF受体家族成员CD 27也参与B细胞调节和增强IG产生。在这份报告中，我们比较了CD 27和CD 40信号在B细胞功能。我们选择性地模拟T细胞辅助的效果，通过添加到用金黄色葡萄球菌科万I菌株和IL-2活化的外周血B细胞中，用CD 70（CD 27配体）基因或CD 154（CD 40配体）基因、单独的载体或CD 70和CD 154基因两者转染的辐射300-19细胞。与CD 40连接诱导的显著增强相比，CD 27连接仅诱导B细胞增殖的轻微增加;双重连接被证明比单独的CD 40连接效率低。相反，IgG产生仅通过单独的CD 27连接增加。此外，当在培养的第2天而不是在第0天给予时，CD 27信号更有效。活化细胞的表型分析显示，CD 27连接增加了显示浆细胞特征（CD 19-、CD 38+）的细胞的百分比，而在CD 40连接后，大多数细胞仍具有生发中心样表型（CD 19+、CD 38+）。我们的研究结果表明，CD 27和CD 40信号不是协同的，而是互补的，涉及T细胞依赖性B细胞活化的不同步骤。当扩增期已经发生时，CD 27在诱导浆细胞分化方面可能更重要。
CD40, a TNF receptor family member, plays a central role in T cell-mediated B cell activation. We have recently demonstrated that CD27, another TNF receptor family member, was also involved in B cell regulation and enhanced Ig production. In this report we compare CD27 and CD40 signals in B cell function. We selectively mimicked the effect of T cell help by addition to peripheral blood B cells activated with Staphylococcus aureus Cowan I strain and IL-2 of irradiated 300-19 cells transfected with either the CD70 (CD27 ligand) gene or the CD154 (CD40 ligand) gene, the vector alone, or both CD70 and CD154 genes. CD27 ligation induced only a slight increase in B cell proliferation compared with the dramatic enhancement induced by CD40 ligation; double ligation proved to be less efficient than CD40 ligation alone. In contrast, IgG production was increased only by CD27 ligation alone. Moreover, the CD27 signal was more efficient when it was given on day 2 of the culture rather than on day 0. Phenotypic analysis of the activated cells showed that CD27 ligation increased the percentage of cells showing a plasma cell profile (CD19-, CD38+), whereas upon CD40 ligation most of the cells still had a germinal center-like phenotype (CD19+, CD38+). Our results suggest that the CD27 and CD40 signals are not synergistic but, rather, are complementary and involve distinct steps of T cell-dependent B cell activation. CD27 may be more important in the induction of plasma cell differentiation at a time when the expansion phase has already occurred.