Changes in Leydig cell activity during the annual testicular cycle of the bat Myotis lucifugus lucifugus: histology and lipid histochemistry.
Changes in Leydig cell activity during the annual testicular cycle of the bat Myotis lucifugus lucifugus: histology and lipid histochemistry.
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DOI：
10.1002/aja.1001780403
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发表时间：
1987-04
期刊：
影响因子：
--
通讯作者：
A. W. Gustafson
中科院分区：
文献类型：
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作者：
A. W. Gustafson
Leydig细胞组织学和睾丸嗜苏丹脂质的变化进行了检查，在蝙蝠鼠耳蝠lucifugus lucifugus（翼手目：Vespertilionidae）在整个年度周期在美国东北部的生精活动。这些变化与血浆睾酮浓度的年度变化相关，最近已被描述为该物种。配子活动发生在5月至8月的几个月，当蝙蝠代谢最活跃。在冬眠期间（10月至4月），精子储存在附睾中，附属腺肥大，生精小管处于静止状态，生殖上皮减少，以保留精原细胞和支持细胞。基于它们的结构和嗜苏丹脂质的循环模式，间质细胞表现出与生精上皮密切相关的活动模式。在春季精子发生恢复期，睾丸间质细胞肥大，脂质内含物增多。这些内含物，在塑料切片中被视为空泡，在冰冻切片中被视为嗜苏丹液滴，在6月下旬达到最大积累。在7月下旬和8月期间，当血液中睾酮水平达到峰值时，脂滴急剧减少，Leydig细胞呈弱嗜苏丹性。9月，当睾酮滴度恢复到低基线水平时，Leydig细胞已经退化，但嗜苏丹包涵体显着增加，其中大部分似乎是脂褐素。在随后的交配和冬眠期间，Leydig细胞退化并充满脂褐素。然而，在觉醒期间，间质细胞变得弱苏丹阳性，而许多大的，强烈的苏丹细胞分散在整个睾丸。在电子显微镜下，这些细胞被鉴定为巨噬细胞。它们似乎通过吞噬间质细胞退化的残留物为新的类固醇生成周期做准备，在每年的睾丸周期中发挥重要作用。在生精小管中也观察到脂质内含物的季节性变化。此外，Leydig细胞周期的雄激素作用和附属器官在这蝙蝠的关系进行了讨论。
Changes in Leydig cell histology and testicular sudanophilic lipids were examined in relation to spermatogenic activity in the bat Myotis lucifugus lucifugus (Chiroptera: Vespertilionidae) throughout the annual cycle in the northeastern United States. These changes were correlated with annual variations in plasma testosterone concentrations which have recently been described for this species. Gametogenic activity occurred during the months of May-August when bats were metabolically most active. During hibernation (October-April), when sperm are stored in the epididymides, and accessory glands are hypertrophic, the seminiferous tubules were at rest, and the germinal epithelium was reduced to reserve spermatogonia and Sertoli cells. Based on their structure and cyclic pattern of sudanophilic lipids, Leydig cells exhibited a pattern of activity that closely paralleled that of the seminiferous epithelium. On renewal of spermatogenesis in spring, Leydig cells became hypertrophied and accumulated lipid inclusions. These inclusions, seen as vacuoles in plastic sections and sudanophilic droplets in frozen sections, reached maximal accumulations in late June. In late July and during August, when peak testosterone levels occur in blood, lipid droplets were dramatically depleted, and Leydig cells were weakly sudanophilic. In September, when testosterone titers return to low baseline levels, Leydig cells had regressed but exhibited a marked increase in sudanophilic inclusions which appeared to be mostly lipofuscins. During the ensuing mating and hibernation periods, Leydig cells were involuted and filled with lipofuscins. During the periarousal period, however, Leydig cells became weakly Sudan-positive while many large, intensely sudanophilic cells were scattered throughout the interstitium. In electron micrographs these cells were identified as macrophages. They appear to play an important role in the annual testicular cycle by phagocytizing the residues of Leydig cell involution in preparation for a new steroidogenic cycle. Seasonal changes in lipid inclusions were also observed in the seminiferous tubules. In addition, the relationship of the Leydig cell cycle to androgen action and the accessory organs in this bat is discussed.