Cloning of a vacuolar H + -pryophosphatase gene from emphemeral plant Olimarabidopsis pumila whose overexpression improve salt tolerance in tobacco
Cloning of a vacuolar H + -pryophosphatase gene from emphemeral plant Olimarabidopsis pumila whose overexpression improve salt tolerance in tobacco
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DOI：
10.5897/ajb2013.13272
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发表时间：
2013-12
影响因子：
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通讯作者：
Fang Xu;Yun-Xia Zhao;F. Wang;Danli Guo;Yan Wei;Xianzhong Huang
中科院分区：
文献类型：
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作者：
Fang Xu;Yun-Xia Zhao;F. Wang;Danli Guo;Yan Wei;Xianzhong Huang
小小寡唇拟南芥是模式植物拟南芥的近缘种，但与拟南芥不同，小寡唇拟南芥是一种耐盐短命植物，广泛分布于中国新疆半干旱半盐碱地区，为耐盐基因挖掘提供了理想的候选植物系统。液泡H ~+ -转运无机焦磷酸酶（V-H ~+ -PPase）是一种产电质子泵，在植物细胞中将质子转运到液泡中起关键作用。从O.在这项研究中，该基因的开放阅读框为2313 bp，编码770个氨基酸，分子量为80.7kDa。OpVP与其他菊科植物V-H + -PPase基因具有较高的氨基酸相似性。结果表明，盐、干旱、阿坝、IAA和GA 3处理均诱导了OpVP基因的表达。OpVP基因的过表达赋予转化烟草增强的耐盐性。转基因烟草在200 mM NaCl存在下生长良好，而野生型植物表现出失绿和生长抑制，甚至死亡。与野生型相比，转基因植株叶片中积累了更多的Na +。此外，转基因植物的叶片在盐胁迫期间保持较高的叶绿素含量。本研究表明，OpVP是一个潜在的耐盐基因，可用于培育耐盐作物。关键词：H + -焦磷酸酶，短小寡唇藻，盐，非生物胁迫.
Olimarabidopsis pumila is a close relative of the model plant Arabidopsis thaliana but, unlike A.thaliana , it is a salt-tolerant ephemeral plant that is widely distributed in semi-arid and semi-salinized regions of the Xinjiang region of China, thus providing an ideal candidate plant system for salt tolerance gene mining. The vacuolar H + -translocating inorganic pyrophosphatase (V-H + -PPase) is an electrogenic proton pump that play pivotal role in translocating protons into vacuoles in plant cells. A V-H + -PPase gene, OpVP , was isolated from O. pumila in this study. The OpVP cDNA has an open reading frame of 2 313 bp, encoding a polypeptide of 770 amino acid residues with an estimated molecular mass of 80.7 kDa. The OpVP shows high amino acid similarity with other Brassicaceae V-H + -PPase genes. Expression profiles under salt and drought treatment, abcisic acid (ABA), indole-3-aceticacid (IAA) and gibberellins (GA) induction were investigated, and the results reveal that the expression of OpVP was induced in leaves under treatment with salt, drought, ABA, IAA and GA3. Overexpression of the OpVP gene confers enhanced salt tolerance to the transformed tobacco. Transgenic tobacco grows well in the presence of 200 mM NaCl, while wild-type plants exhibit chlorosis and growth inhibition, even death. Compared with wild-type, transgenic plants accumulated more Na + in leaves. Moreover, the leaves of transgenic plants retain higher chlorophyll content during salt stress. This study shows that OpVP is a potential gene for salt tolerance, and can be used in future for developing salt tolerant crops. Key words: H + -pyrophoshatase, Olimarabidopsis pumila , salt, abiotic stress.