Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference
Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference
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DOI：
10.1016/j.jbiotec.2004.02.019
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发表时间：
2004-08-05
影响因子：
4.1
通讯作者：
Kobayashi, A
中科院分区：
文献类型：
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作者：
Fukusaki, E;Kawasaki, K;Kobayashi, A
抑制与花色形成相关的生物合成基因是获得目标花色的重要途径。在这里，我们报告的花色的园林植物蓝猪耳成功地调制RNA干扰（RNAi）对查尔酮合成酶（CHS），花色素苷和黄酮类化合物的生物合成的关键酶基因。通过使用CHS mRNA的编码区和3 '-非翻译区中的每一个作为RNAi靶标，成功地诱导了彻底的和基因特异性的基因沉默，并且将原始蓝色花的颜色分别调制为白色和淡颜色。我们的研究结果表明，RNAi是非常有用的调制花色的商业重要的园林植物。(C)2004 Elsevier B. V.保留所有权利。
Suppression of biosynthetic genes involved in flower color formation is an important approach for obtaining target flower colors. Here we report that flower color of the garden plant Torenia hybrida was successfully modulated by RNA interference (RNAi) against a gene of chalcone synthase (CHS), a key enzyme for anthocyanin and flavonoid biosynthesis. By using each of the coding region and the 3'-untranslated region of the CHS mRNA as an RNAi target, exhaustive and gene-specific gene silencing were successfully induced, and the original blue flower color was modulated to white and pale colors, respectively. Our results indicate that RNAi is quite useful for modulations of flower colors of commercially important garden plants. (C) 2004 Elsevier B.V. All rights reserved.