Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM
Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM
复制标题
家蚕两种微孢子虫微孢子虫CQ1和Vairimorpha necatrix BM感染家蚕的病理分析
DOI：
10.1016/j.jip.2017.12.005
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发表时间：
2018-03-01
影响因子：
3.4
通讯作者：
Zhou, Ze-Yang
中科院分区：
文献类型：
--
作者：
Meng, Xian-Zhi;Luo, Bo;Zhou, Ze-Yang
家蚕微孢子虫CQ 1能在家蚕体内垂直传播，而毒蜥虫BM不能。因此，这两种微孢子虫感染家蚕的病理差异需要澄清。在此，我们比较了N. Bombycis CQ 1和V.necatrix BM对家蚕的抗性。采用石蜡切片、扫描电镜和透射电镜观察肠道、睾丸和卵巢的病理学特征。结果表明，线虫弧菌BM的毒力弱于线虫N. Bombycis CQ 1.其次，钩虫BM感染的典型症状是在肠道后部产生充满不同阶段病原体的异种瘤。而感染N. Bombycis CQ 1.第三，N. Bombycis CQ 1在感染卵巢时可使孢子聚集在气管附近。值得注意的是，N.家蚕CQ 1感染卵巢上皮细胞和结缔组织，而V.necatrix BM不感染。虽然线虫弧菌BM在体内不能感染家蚕卵巢，但它可以在体外感染胚胎和卵巢细胞系。本研究首次报道了我国北方稻瘟病菌侵染特性的比较。Bombycis CQ 1和V.necatrix BM在家蚕组织中的致病性差异，为解释这两种微孢子虫不同的传播策略提供了详细而直观的病理学特征信息。
Microsporidia Nosema bombycis CQ1 can be vertically transmitted in silkworm Bombyx mori but Vairimorpha necatrix BM cannot. Therefore, the pathological differences in silkworm infected with these two microsporidia required clarification. Here, we compared the virulence of N. bombycis CQ1 and V. necatrix BM against silkworm. The pathological characteristics in intestine, testis and ovary were surveyed using paraffin sections, scanning electron microscopy and transmission electron microscopy. Our data firstly showed that the virulence of V. necatrix BM was weaker than that of N. bombycis CQ1. Secondly, the typical symptom of V. necatrix BM infection is making xenomas, which are full of pathogens in different stages, at the posterior of intestine. However, no xenomas were formed surrounding intestines infected with N. bombycis CQ1. Thirdly, N. bombycis CQ1 can cluster spores near the trachea while infecting ovaries. It is worth noting that N. bombycis CQ1 infected epithelial cells and connective tissues of ovaries, while V. necatrix BM did not. Although silkworm ovaries can not be infected by V. necatrix BM in vivo, it can infect embryonic and ovarian cell lines in vitro. This study is the first report about comparing infection features of N. bombycis CQ1 and V. necatrix BM in silkworm tissues and it provided elaborate and visual information of pathological characteristics which can help to explain the different transmission strategies of these two microsporidia.