Tea Polyphenol-Reduced Graphene Oxide Deposition on Titanium Surface Enhances Osteoblast Bioactivity
Tea Polyphenol-Reduced Graphene Oxide Deposition on Titanium Surface Enhances Osteoblast Bioactivity
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DOI：
10.1166/jnn.2018.14649
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发表时间：
2018-05-01
影响因子：
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通讯作者：
Cai, Xiaoxiao
中科院分区：
文献类型：
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作者：
Liu, Mengting;Hao, Liying;Cai, Xiaoxiao
石墨烯是一种新型的碳基材料，因其具有促进成骨细胞增殖的潜在作用而被广泛用作成骨剂。茶多酚还原氧化石墨烯（TPG）是制备石墨烯的一种简单、环保的原料。本研究将TPG沉积在钛基体上，以促进骨再生。采用酸碱预处理法制备了蜂窝状结构的Ti-TPG复合材料，并通过电化学沉积法在其表面固定了TPG层。利用扫描电子显微镜（SEM）、原子力显微镜（AFM）和X射线衍射（XRD）对TPG在钛表面的固定化进行了表征。此外，评价了Ti-TPG表面对大鼠成骨细胞的生物学反应。并对细胞在标本上的粘附、增殖及成骨相关基因的表达进行了研究。结果表明，Ti-TPG在调节细胞活性方面优于钛合金，Ti-TPG有望成为生物材料的理想涂层。
Graphene, a novel carbon-based material, has been widely used as osteogenic agent for the potential effect on the promotion of osteoblast proliferation. Tea polyphenol-reduced graphene oxide (TPG) is a simple and environmental-friendly raw material to obtain graphene. In this study, TPG was deposited on the Ti substrate to promote the bone regeneration. We prepared a honeycomb-like structure by acid and alkali pretreatment and immobilized the TPG layer (Ti-TPG) on the surface via electrochemical deposition. Scanning electron microscopy (SEM), atomic force microscopy (AFM) and X-ray diffraction (XRD) were used to identify the immobilization of TPG on the titanium (Ti) successfully. Furthermore, the biological response of the Ti-TPG surface to rat osteoblast was evaluated. We also studied the cell adhesion, proliferation and expression of ossification genes on the sample. The results revealed that Ti-TPG had an advantage over Ti alloys in modulating cellular activity and Ti-TPG may be a promising coating for biological materials.