Live imaging and modeling for shear stress quantification in the embryonic zebrafish heart
Live imaging and modeling for shear stress quantification in the embryonic zebrafish heart
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DOI：
10.1016/j.ymeth.2015.09.017
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发表时间：
2016-02-01
期刊：
影响因子：
4.8
通讯作者：
Vermot, Julien
中科院分区：
文献类型：
--
作者：
Boselli, Francesco;Vermot, Julien
血流动力学切应力由构成心脏内细胞层的内皮细胞感知，并在心脏形态发生中起主要作用。然而，潜在的血流动力学和相关的机械刺激所经历的内皮细胞仍然知之甚少。由于需要高时间分辨率成像，使得能够分辨在跳动的心脏中产生的流动轮廓，该领域的进展受到阻碍。为了填补这一空白，我们提出了一种方法来分析壁动力学，流场和壁剪切应力的发展斑马鱼心脏。该方法结合了实时共焦成像和计算流体动力学，以克服与发育中心脏中血流的实时成像相关的困难。为了提供该方法的适用性的一个例子，我们讨论了血流动力学的频率内容所感知的内皮细胞在瓣膜形成的开始，以及如何的基本频率的壁剪切应力代表一个独特的机械提示内皮细胞，心脏瓣膜的前体。(C)2015年，作者。爱思唯尔公司出版。
Hemodynamic shear stress is sensed by the endocardial cells composing the inner cell layer of the heart, and plays a major role in cardiac morphogenesis. Yet, the underlying hemodynamics and the associated mechanical stimuli experienced by endocardial cells remains poorly understood. Progress in the field has been hampered by the need for high temporal resolution imaging allowing the flow profiles generated in the beating heart to be resolved. To fill this gap, we propose a method to analyze the wall dynamics, the flow field, and the wall shear stress of the developing zebrafish heart. This method combines live confocal imaging and computational fluid dynamics to overcome difficulties related to live imaging of blood flow in the developing heart. To provide an example of the applicability of the method, we discuss the hemodynamic frequency content sensed by endocardial cells at the onset of valve formation, and how the fundamental frequency of the wall shear stress represents a unique mechanical cue to endocardial, heart-valve precursors. (C) 2015 The Authors. Published by Elsevier Inc.