Norbixin ingestion did not induce any detectable DNA breakage in liver and kidney but caused a considerable impairment in plasma glucose levels of rats and mice
Norbixin ingestion did not induce any detectable DNA breakage in liver and kidney but caused a considerable impairment in plasma glucose levels of rats and mice
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DOI：
10.1016/s0955-2863(02)00177-8
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发表时间：
2002-07-01
影响因子：
5.6
通讯作者：
Kovary, K
中科院分区：
文献类型：
--
作者：
Fernandes, ACS;Almeida, CA;Kovary, K
从Bixa orellana的种子中提取的类胡萝卜素bixin和norbixin已被广泛用于着色食品。在这项研究中，通过饮用水摄入21天后，在小鼠和大鼠中研究了纯化或未纯化的降胭脂树红（含50%降胭脂树红的胭脂树红提取物）的毒性。将小鼠暴露于56和351 mg/kg（胭脂树提取物）以及0.8、7.6、66和274 mg/kg（降胭脂树橙）的剂量。大鼠暴露于0.8、7.5和68 mg/kg（胭脂树红提取物）和0.8、8.5和74 mg/kg（降胭脂树红）的剂量。在大鼠中，通过血浆化学未检测到毒性。降胭脂树红使小鼠血浆丙氨酸氨基转移酶（ALT）活性升高，而降胭脂树红和胭脂树红提取物均使血浆总蛋白和球蛋白含量降低（P < 0.05）。然而，通过组织病理学分析未在肝脏中检测到毒性体征。用彗星试验评价，在用胭脂树红色素处理的小鼠的肝脏或肾脏中没有检测到DNA断裂的增强。然而，降胭脂树橙对两种啮齿动物的呼吸均有显著影响。在大鼠中，降胭脂树橙诱导高血糖，其范围从高于对照水平的26.9%（8.5mg/kg降胭脂树橙，至52.6%（74 mg/kg降胭脂树橙，P < 0.01）。在小鼠中，降胭脂树橙诱导的低血糖范围为低于对照水平的144%（0.8 mg/kg降胭脂树橙，P < 0.05）至21.5%（66 mg/kg降胭脂树橙，P < 0.001）。用胭脂树红色素处理的大鼠和小鼠分别显示高胰岛素血症和低胰岛素血症，表明胰腺β细胞是功能性的。应进行更多的研究，以充分了解种属相关差异如何影响降胭脂树橙的生物命运。(C)2002年爱思唯尔科技有限公司All rights reserved.
From the seeds of Bixa orellana are extracted the carotenoids bixin and norbixin that have been widely used for coloring food. In this study, the toxicity of norbixin, purified or not (annatto extract containing 50% norbixin), was investigated in mice and rats after 21 days of ingestion through drinking water. Mice were exposed to doses of 56 and 351 mg/kg (annatto extract) and 0.8, 7.6, 66 and 274 mg/kg (norbixin). Rats were exposed to doses of 0.8, 7.5 and 68 mg/kg (annatto extract) and 0.8, 8.5 and 74 mg/kg (norbixin). In rats, no toxicity was detected by plasma chemistry. In mice, norbixin induced an increase in plasma alanine aminotransferase activity (ALT) while both norbixin and annatto extract induced a decrease in plasma total protein and globulins (P < 0.05). However, no signs of toxicity were detected in liver by histopathological analysis. No enhancement in DNA breakage was detected in liver or kidney from mice treated with annatto pigments, as evaluated by the comet assay. Nevertheless, there was a remarkable effect of norbixin on the glycemia of both rodent species. In rats, norbixin induced hyperglycemia that ranged from 26.9% (8.5 mg/kg norbixin, to 52.6% (74 mg/kg norbixin, P < 0.01) above control levels. In mice, norbixin induced hypoglycemia that ranged from 144% (0.8 mg/kg norbixin, P < 0.05) to 21.5% (66 mg/kg norbixin, P < 0.001) below control levels. Rats and mice treated with annatto pigments showed hyperinsulinemia and hypoinsulinemia, respectively indicating that pancreatic beta-cells were functional. More studies should be performed to fully understand of how species-related differences influences the biological fate of norbixin. (C) 2002 Elsevier Science Inc. All rights reserved.