LOW-DENSITY NEUTROPHILS IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS, RHEUMATOID-ARTHRITIS, AND ACUTE RHEUMATIC-FEVER
LOW-DENSITY NEUTROPHILS IN PATIENTS WITH SYSTEMIC LUPUS-ERYTHEMATOSUS, RHEUMATOID-ARTHRITIS, AND ACUTE RHEUMATIC-FEVER
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DOI：
10.1002/art.1780291105
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发表时间：
1986-11-01
影响因子：
--
通讯作者：
KAJDACSYBALLA, A
中科院分区：
文献类型：
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作者：
HACBARTH, E;KAJDACSYBALLA, A
系统性红斑狼疮、类风湿性关节炎和急性风湿热患者外周血单个核细胞的Ficoll-Hypaque密度梯度制剂被低浮力密度中性粒细胞高度“污染”。来自这些患者的血浆可以诱导具有正常浮力密度的中性粒细胞的浮力的体外降低。补体激活血清和聚集的丙种球蛋白也能引起类似的变化。这些数据表明，活化的中性粒细胞是这些患者外周血中的常见发现，并可能影响对这些细胞的任何研究的解释。通过Ficoll-Hypaque梯度分离的淋巴细胞的功能研究还应考虑风湿性疾病患者细胞制剂的较高程度的杂质。
Ficoll-Hypaque density gradient preparations of peripheral blood mononuclear cells from patients with systemic lupus erythematosus, rheumatoid arthritis, and acute rheumatic fever were highly "contaminated" with low buoyant density neutrophils. Plasma from these patients could induce an in vitro decrease of buoyancy in neutrophils with normal buoyant density. Similar change could be induced by complement-activated sera and aggregated gamma globulin. These data suggest that activated neutrophils are a common finding in the peripheral blood of these patients and may influence the interpretation of any studies with these cells. Functional studies of lymphocytes separated by Ficoll-Hypaque gradients should also take into account the higher degree of impurity of the cell preparations in patients with rheumatic diseases.