Determination of different arsenic species in food grade spirulina powder by ion chromatography combined with inductively coupled plasma mass spectrometry
Determination of different arsenic species in food grade spirulina powder by ion chromatography combined with inductively coupled plasma mass spectrometry
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离子色谱-电感耦合等离子体质谱法测定食品级螺旋藻粉中不同形态的砷
DOI：
10.1002/jssc.201700618
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发表时间：
2017
影响因子：
3.1
通讯作者：
Yang GuiDi
中科院分区：
文献类型：
--
作者：
Li XiangMei;Chen YuXi;Ye Jun;Fu FengFu;Pokhrel Ganga Raj;Zhang Huang;Zhu YongGuan;Yang GuiDi
建立了“两步”加压微波辅助萃取-离子色谱-电感耦合等离子体质谱法测定螺旋藻样品中不同形态砷的方法。该方法以H2 O2/H2O（1：5，v/v）为溶剂，除亚砷酸盐外，其余砷形态均采用水为溶剂萃取。该方法提取时间短（20.0min），回收率>96%。本法用于螺旋藻样品中砷的分离和测定，回收率为84-105%，相对标准偏差为2- 4%。采用优化后的方案对来自中国7个省（内蒙古、浙江、福建、海南、云南、江苏和广西）的食品级螺旋藻粉样品进行了分析。在所有螺旋藻样品中检测到的砷酸盐浓度范围为170-394 ng/g。除广西外，上述六个省份的螺旋藻中均检出二甲基胂酸，浓度范围为32-839 ng/g。单甲基胂酸（67 ± 3 ng/g）仅在云南产螺旋藻中检出。除海南和广西外，其余5个省的螺旋藻中均检出砷，砷含量范围为28-147 ng/g。除广西外，在上述6个省的螺旋藻中还发现了5种未知有机砷形态。
A “two‐step” pressurized microwave‐assisted extraction method coupled with ion chromatography with inductively coupled plasma mass spectrometry for the determination of different arsenic species in spirulina samples was developed. The extraction method used H2O2/H2O (1:5, v/v) as solvent to extract all arsenic species except arsenite, which was extracted by using water as solvent. The extraction method had a satisfactory recovery (>96%) and took a short time (20.0 min). With our method, all arsenic species in spirulina samples were completely separated and determined with recoveries of 84–105% and relative standard deviations of 2–4%. Food‐grade spirulina powder samples from seven provinces (Inner Mongolia, Zhejiang, Fujian, Hainan, Yunnan, Jiangsu, and Guangxi) in China were analyzed using the optimized protocol. Arsenate was detected at the concentration range of 170–394 ng/g in all the spirulina samples. Dimethylarsinic acid was detected at the concentration range of 32–839 ng/g in spirulina from above‐six provinces except Guangxi. Monomethylarsonic acid (67 ± 3 ng/g) was detected only in spirulina from Yunnan province. Arsenite was detected at the concentration range of 28–147 ng/g in spirulina from above five provinces except Hainan and Guangxi. Five unknown organic arsenic species were found in spirulina from above six provinces except Guangxi.