EFFICIENT TRANSCRIPTION OF A PROTEIN-CODING GENE FROM THE RNA POLYMERASE-I PROMOTER IN TRANSFECTED CELLS
EFFICIENT TRANSCRIPTION OF A PROTEIN-CODING GENE FROM THE RNA POLYMERASE-I PROMOTER IN TRANSFECTED CELLS
复制标题
DOI：
10.1073/pnas.82.3.722
复制
发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
SKINNER, JA
中科院分区：
文献类型：
--
作者：
GRUMMT, I;SKINNER, JA
将小鼠核糖体启动子与编码氯霉素乙酰转移酶（CAT）的基因融合并转染小鼠细胞后，检测其活性。合成了非常少的CAT酶，但在核糖体DNA起始位点正确起始的高水平CAT特异性RNA。特异性转录物的量既不受上游间隔序列的长延伸的影响，也不受莫洛尼鼠肉瘤病毒增强子的插入的影响。缺失突变体pMr Δ- 39，其在体外显示出完全活性，在体内表现出90%的模板活性降低。核糖体DNA的22个碱基对（位置-35和-14之间）被外源DNA序列取代的突变体被证明是转录失活的。融合基因仅在小鼠细胞中转录，表明种特异性转录因子参与核糖体启动子识别。
The activity of the mouse ribosomal promoter was examined after fusion to the gene coding for chloramphenicol acetyltransferase (CAT) and transfection into mouse cells. Very little CAT enzyme but high levels of CAT-specific RNA correctly initiated at the ribosomal DNA start site were synthesized. The amount of specific transcripts was neither influenced by long stretches of upstream spacer sequences nor by the insertion of the Moloney murine sarcoma virus enhancer. The deletion mutant pMr.DELTA.-39, which was shown to be fully active in vitro, exhibited a 90% decrease in template activity in vivo. A mutant in which 22 base pairs of ribosomal DNA (between positions -35 and -14) were substituted by foreign DNA sequences proved transcriptionally inactive. The fusion genes were only transcribed in mouse cells, indicating that species-specific transcription factors are involved in ribosomal promoter recognition.