A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis
A glycine-rich RNA-binding protein affects gibberellin biosynthesis in Arabidopsis
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DOI：
10.1007/s11033-013-2878-7
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发表时间：
2013
影响因子：
2.8
通讯作者：
B. Löhr;C. Streitner;A. Steffen;T. Lange;D. Staiger
中科院分区：
文献类型：
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作者：
B. Löhr;C. Streitner;A. Steffen;T. Lange;D. Staiger
RNA结合蛋白拟南芥富含甘氨酸的RNA结合蛋白7(AtGRP7)调节着众多靶转录本的稳态丰度。塔里安娜。在这里，我们发现编码赤霉素生物合成途径的第一个酶的GA_1和GA_2转录本在异地过度表达AtGRP7的转基因植物(AtGRP7-OX植物)中以低水平表达。此外，在AtGRP7-OX植物中，生物活性植物激素GA4以及GA生物合成途径的几个中间体的水平降低。转基因植物的营养茎长度有所缩短。外源GA的应用在很大程度上逆转了植株的表型，增加了营养间节的数量。AtGRP7-OX植株开花时叶片比wt植株少，这表明AtGRP7-OX植株的开花促进作用绕过了GA水平降低的影响。在GA处理下，AtGRP7-OX植株的开花时间仅略早于野生型植株。因此，外源GA在减少AtGRP7-OX植株开花时的叶片数方面只有很小的附加效应。
The RNA-binding proteinArabidopsis thalianaglycine-rich RNA-binding protein 7 (AtGRP7) regulates the steady-state abundance of numerous target transcripts inA. thaliana. Here we show that theGA1andGA2transcripts encoding the first enzymes of the gibberellin biosynthetic pathway are expressed at reduced levels in transgenic plants ectopically over-expressingAtGRP7 (AtGRP7-ox plants). Furthermore, the levels of the bioactive phytohormone GA4as well as of several intermediates of the GA biosynthetic pathway are reduced inAtGRP7-ox plants. The transgenic plants show a reduced length of the vegetative stem. The application of exogenous GA largely reverses the phenotype by increasing the number of vegetative internodes.AtGRP7-ox plants flower with fewer leaves than wt plants, suggesting that the floral promotive effect ofAtGRP7 bypasses the effect of a reduced GA level inAtGRP7-ox plants. Upon GA treatment,AtGRP7-ox plants flower only slightly earlier than wild type plants. Thus, exogenous GA has only a small additional effect in reducing the number of leaves at the onset of flowering inAtGRP7-ox plants.