Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot
Hepatocyte-derived fibrinogen-related protein-1 is associated with the fibrin matrix of a plasma clot
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DOI：
10.1016/j.bbrc.2006.09.018
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发表时间：
2006-11-10
影响因子：
3.1
通讯作者：
Leebeek, F. W. G.
中科院分区：
文献类型：
--
作者：
Rijken, D. C.;Dirkx, S. P. G.;Leebeek, F. W. G.
为了研究纤维蛋白原和纤维蛋白的多种功能，我们正在研究哪些蛋白质结合到血浆凝块的纤维蛋白基质，通过使用蛋白质组学方法。通过2-D凝胶电泳分析来自洗涤的血浆凝块的提取物。经MALDI-TOF分析、分子量（34 kDa）、等电点（pI 5.5）以及Western blot分析，鉴定出一个相对丰富的蛋白点为肝细胞源性纤维蛋白原相关蛋白-1（HFREP-1）。血浆中的HFREP-1在凝块形成期间几乎完全结合到纤维蛋白基质。几种纯化的纤维蛋白原制剂被证明被HFREP-1污染。它的结论是HFREP-I（也称为hepassocin），一种具有肝细胞生长调节特性的蛋白质，发生在血浆中，并与纤维蛋白和可能的纤维蛋白原密切相关。(c)2006年爱思唯尔公司All rights reserved.
In order to study the multiple functions of fibrinogen and fibrin, we are investigating which proteins bind to the fibrin matrix of a plasma clot by using a proteomic approach. Extracts from washed plasma clots were analysed by 2-D gel electrophoresis. A relatively abundant spot was identified as hepatocyte-derived fibrinogen-related protein-1 (HFREP-1) by MALDI-TOF analysis, molecular mass (34 kDa), iso-electric point (pI 5.5) as well as by Western blot analysis. HFREP-1 in plasma almost completely bound to the fibrin matrix during clot formation. Several purified fibrinogen preparations proved to be contaminated with HFREP-1. It is concluded that HFREP-I (also named hepassocin), a protein with liver cell growth regulatory properties, occurs in plasma and strongly associates with fibrin and possibly fibrinogen. (c) 2006 Elsevier Inc. All rights reserved.