Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction.
Typing of Y chromosome single nucleotide polymorphisms in a Japanese population by a multiplexed single nucleotide primer extension reaction.
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DOI：
10.1016/s1344-6223(02)00032-9
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发表时间：
2002-09-01
期刊：
影响因子：
--
通讯作者：
Ishizu, Hideo
中科院分区：
文献类型：
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作者：
Inagaki, Sachiyo;Yamamoto, Yuji;Ishizu, Hideo
我们开发了一种基于多重单核苷酸引物延伸的人类Y染色体上单核苷酸多态性（SNP）分型的新方法。该方法的优点是可以同时快速分型多个SNP。我们检测了159名日本男性Y染色体上的15个不同SNP位点，M9，M105，M122，M125，M128，M130，SRY 465，IMS-JST 006241，IMS-JST 006841，IMS-JST 002611，IMS-JST 003305，IMS-JST 008425，IMS-JST 021354，IMS-JST 021355和IMS-JST 055457。从这15个位点的分型结果中，我们发现了13种单倍型。每个位点的基因多样性范围为0.025至0.486，单倍型多样性估计为0.838。这种方法可以很容易地应用于个人身份识别和亲子鉴定。
We have developed a new method for typing single nucleotide polymorphisms (SNPs) on the human Y chromosome based on a multiplexed single nucleotide primer extension. This method has the advantage that several SNPs are typed rapidly and simultaneously. We examined 15 different SNP loci on Y chromosome, M9, M105, M122, M125, M128, M130, SRY465, IMS-JST006241, IMS-JST006841, IMS-JST002611, IMS-JST003305, IMS-JST008425, IMS-JST021354, IMS-JST021355 and IMS-JST055457, in 159 Japanese males. From the typing results of these 15 loci, we found 13 haplotypes. Gene diversity for each locus ranged from 0.025 to 0.486 and the haplotype diversity was estimated to be 0.838. This method could be readily applied for personal identification and paternity testing.