Structural characterization of Haemophilus parainfluenzae lipooligosaccharide and elucidation of its role in adherence using an outer core mutant.
Structural characterization of Haemophilus parainfluenzae lipooligosaccharide and elucidation of its role in adherence using an outer core mutant.
复制标题
副流感嗜血杆菌脂寡糖的结构表征及其在使用外核突变体粘附中的作用的阐明。
DOI：
10.1139/w08-082
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发表时间：
2008
影响因子：
2.8
通讯作者：
Cox,Andrew
中科院分区：
文献类型：
--
作者：
Pollard,Angela;StMichael,Frank;Connor,Lynn;Nichols,Wade;Cox,Andrew
副流感嗜血杆菌（Haemophilus parainfluenzae）是一种存在于人类口咽部的革兰氏阴性细菌，属于巴斯德菌科，与森氏嗜血杆菌（Haemophilus sengis）和放线杆菌（Actinobacillus）关系最为密切。表面展示的脂寡糖的表征已经鉴定出在粘附中至关重要的组分。我们检测了2株副流感嗜血杆菌临床分离株脂寡糖的寡糖结构。通过标准方法从纯化的脂寡糖中分离核心寡糖。通过单糖和甲基化分析、核磁共振光谱法和质谱法的组合建立了结构信息，揭示了以下结构：R-（1-6）-β-Glc-（1-4）-d，d-α-Hep-（1-6）-β-Glc-（1-4）-取代L的三庚糖-Kdo内核，d-α-Hep-（1-2）-l，d-α-Hep-（1-3）-l，在Kdo的近端d-α-Hep残基的4-位上具有d-α-Hep-（1-5）-α-Kdo，并且在中心d-α-Hep残基的6-位上具有PEtn残基。在菌株4282中，R取代基是β-半乳糖，并且在菌株4201中，在远端葡萄糖处没有取代基。 这些分析表明，副流感嗜血杆菌脂寡糖的多个结构方面与无法分型的流感嗜血杆菌脂寡糖相当。本研究还鉴定了菌株4201中的半乳聚糖和菌株4282中的葡聚糖。副流感嗜血杆菌显示出与不可分型流感嗜血杆菌相同的粘附于支气管上皮细胞系的程度。然而，缺乏脂寡糖外核的副流感嗜血杆菌突变株显示对上皮细胞的粘附减少，表明副流感嗜血杆菌脂寡糖在组织定植中起作用。
The opportunistic pathogen Haemophilus parainfluenzae is a gram-negative bacterium found in the oropharynx of humans.Haemophilus parainfluenzaeis a member of thePasteurellaceaefamily in which it is most closely related to Haemophilus sengis and Actinobacillus. Characterization of surface displayed lipooligosaccharide has identified components that are crucial in adherence. We examined the oligosaccharide structure of lipooligosaccharide from 2 clinical isolates ofH. parainfluenzae. Core oligosaccharide was isolated by standard methods from purified lipooligosaccharide. Structural information was established by a combination of monosaccharide and methylation analyses, nuclear magnetic resonance spectroscopy, and mass spectrometry revealing the following structures: R-(1-6)-β-Glc-(1-4)-d,d-α-Hep-(1-6)-β-Glc-(1-4)- substituting a tri-heptose-Kdo inner core of L,d-α-Hep-(1-2)-l,d-α-Hep-(1-3)-l,d-α-Hep-(1-5)-α-Kdo at the 4-position of the proximall,d-α-Hep residue to Kdo, and with a PEtn residue at the 6-position of the centrall,d-α-Hep residue. In strain 4282, the R substituent is β-galactose and in strain 4201 there is no substituent at the distal glucose. These analyses have revealed that multiple structural aspects ofH. parainfluenzaelipooligosaccharide are comparable with nontypeable Haemophilus influenzae lipooligosaccharide. This study also identified a galactan in strain 4201 and a glucan in strain 4282.Haemophilus parainfluenzaewas shown to adhere to a bronchial epithelial cell line to the same degree as nontypeableH. influenzae. However, anH. parainfluenzaemutant lacking the outer core of the lipooligosaccharide showed diminished adherence to the epithelial cells, suggesting thatH. parainfluenzaelipooligosaccharide plays a role in tissue colonization.