Neutral core oligosaccharides of bovine submaxillary mucin -: Use of lead tetraacetate in the cold for establishing branch positions
Neutral core oligosaccharides of bovine submaxillary mucin -: Use of lead tetraacetate in the cold for establishing branch positions
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DOI：
10.1046/j.1432-1327.1998.2580603.x
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发表时间：
1998-12-01
期刊：
影响因子：
--
通讯作者：
Bendiak, B
中科院分区：
文献类型：
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作者：
Mårtensson, S;Levery, SB;Bendiak, B
通过碱性硼氢化物处理，从牛颌下粘蛋白中释放寡糖糖醇。通过HPLC分离含有较小中性寡糖的级分，以得到单糖-、二糖-和三糖-糖醇大小的19种不同结构，以及三种岩藻糖基化四糖醇。分子通过NMR光谱、电喷雾-质谱-质谱、全甲基化分析和在-30 ° C下的高选择性Pb（0Ac）（4）氧化、随后硼氢化物还原来鉴定。发现Pb（OAc）氧化通常适用于鉴定所有核心结构的取代的分支位置。在分离的寡糖糖醇的结构之前没有报告，包括12个结构没有报告的牛颌下粘蛋白，和4个结构（三个核心结构）中没有发现的Carbbank数据库。
Oligosaccharide alditols were released from bovine submaxillary mucin by alkaline borohydride treatment. The fractions containing smaller neutral oligosaccharides were separated by HPLC, to give, in the mono-, di- and trisaccharide-alditol sizes, 19 different structures, in addition to three fucosylated tetrasaccharide alditols. Molecules were identified by NMR spectroscopy, electrospray-mass-spectrometry - mass-spectrometry, permethylation analyses, and highly selective Pb(OAc)(4) oxidation at -30 degrees C, followed by borohydride reduction. Pb(OAc), oxidation was found to be generally applicable in identifying the branch position(s) of substitutions for all core structures. Among the isolated oligosaccharide alditols were structures not previously reported, including 12 structures not reported from bovine submaxillary mucin, and four structures (three core structures) not found in the Carbbank database.