FTIR spectroscopy of metalloproteins.
FTIR spectroscopy of metalloproteins.
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金属蛋白的 FTIR 光谱。
DOI：
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发表时间：
2014
影响因子：
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通讯作者：
A. L. De Lacey
中科院分区：
文献类型：
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作者：
Óscar Gutiérrez‐Sanz;O. Rüdiger;A. L. De Lacey
蛋白质对红外辐射的吸收提供了有关其结构和功能的重要信息。最强的红外带对应于所有肽键吸收的重叠。此外，在许多金属蛋白中，它们的辅基具有内在配体或结合在红外线中强烈吸收的底物/抑制剂。本文以氢化酶为例，详细介绍了几种傅里叶变换红外光谱法用于研究金属蛋白质的结构-功能关系。
Absorption of infrared radiation by proteins gives important information about their structure and function. The most intense infrared bands correspond to the overlap of all the peptide bond absorption. Additionally, in many metalloproteins their prosthetic groups have intrinsic ligands or bind substrates/inhibitors that absorb intensively in the infrared. Here, we describe thoroughly several Fourier transform infrared methods for studying structure-function relationships in metalloproteins, using hydrogenases as an example.