Epibrassinolide induces changes in indole-3-acetic acid, abscisic acid and polyamine concentrations and enhances antioxidant potential of radish seedlings under copper stress
Epibrassinolide induces changes in indole-3-acetic acid, abscisic acid and polyamine concentrations and enhances antioxidant potential of radish seedlings under copper stress
复制标题
DOI：
10.1111/j.1399-3054.2010.01403.x
复制
发表时间：
2010-11-01
影响因子：
6.4
通讯作者：
Kanwar, Mukesh
中科院分区：
文献类型：
--
作者：
Choudhary, Sikander Pal;Bhardwaj, Renu;Kanwar, Mukesh
研究了表油菜素内酯（EBL）对7日龄莴苣组织中吲哚-3-乙酸（IAA）、脱落酸（ABA）和多胺（PA）浓度及抗氧化能力的影响。对铜胁迫下生长的“Pusa chetki”幼苗进行了研究。与单独处理金属相比，单独处理EBL或与Cu联合处理可提高游离和结合IAA滴度。单独处理EBL后，游离ABA和结合ABA含量略有增加。然而，与单独使用Cu相比，EBL与Cu联合使用导致两种形式的ABA均显著增加。在分析的PAs中，仅腐胺和尸胺浓度通过EBL单独处理而增强。相比之下，EBL + Cu处理的幼苗腐胺和精胺含量显著下降。与金属处理和对照处理相比，EBL单独处理或与Cu联合处理提高了愈创木酚过氧化物酶（EC1.11.1.7）、过氧化氢酶（EC 1.11.1.6）、超氧化物歧化酶（EC 1.15.1.1）和谷胱甘肽还原酶（EC 1.6.4.2）的活性和蛋白质含量。添加或不添加Cu的EBL处理也记录了丙二醛含量的显著降低。在单独使用EBL或与Cu联合处理的幼苗中，植物螯合素含量也有所增加。利用DPPH（1,1-二苯基苦酰基肼）进行抗氧化活性测定，证明了自由基清除能力的显著提高，脱氧核糖和还原能力的提高，以及抗坏血酸、总酚和脯氨酸含量的增加，也表明EBL应用对减轻铜诱导的萝卜幼苗氧化应激有重要影响。
In the present study, the effects of epibrassinolide (EBL) on indole-3-acetic acid (IAA), abscisic acid (ABA) and polyamine (PA) tissue concentrations and antioxidant potential of 7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress were investigated. EBL treatment alone or in combination with Cu enhanced free and bound IAA titers when compared with the metal alone. Modest increases in free and bound ABA contents were observed for EBL treatment alone. However, the combination of EBL with Cu caused major increases in both forms of ABA, over Cu alone. Among the PAs analyzed, only putrescine and cadaverine concentrations were enhanced by EBL treatment alone. By contrast, a significant decline in putrescine and spermine contents was found in seedlings treated with EBL plus Cu. EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC 1.11.1.6), superoxide dismutase (EC 1.15.1.1) and glutathione reductase (EC 1.6.4.2) and protein contents in comparison with metal and control treatments. A major decrease in malondialdehyde content was also recorded for EBL treatments with or without Cu. An increase in phytochelatin content was also observed in seedlings treated with EBL alone or in combination with Cu. Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings.