Effect of diet composition on coenzyme A and its thioester pools in various rat tissues
Effect of diet composition on coenzyme A and its thioester pools in various rat tissues
复制标题
饮食成分对不同大鼠组织中辅酶A及其硫酯库的影响
DOI：
10.1016/j.bbrc.2012.06.037
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发表时间：
2012
影响因子：
3.1
通讯作者：
長南茂
中科院分区：
文献类型：
--
作者：
徳竹由華;飯尾恒;鬼澤直樹;緒形雄太;小針大助;豊田淳;長南茂
本文分析了13种禁食大鼠组织中3种辅酶A(CoA)的分子形态，即乙酰辅酶A、丙二酰辅酶A和非酯化辅酶A。在延髓、肝脏、心脏和棕色脂肪组织中观察到相对较大的总辅酶A池大小，包括乙酰辅酶A、丙二酰辅酶A和辅酶A。聚焦于CoA池大小的变化响应给定饮食的营养成分，连续喂食高脂饮食4周的大鼠的总CoA池显著高于高碳水化合物或高蛋白饮食的大鼠，下丘脑、小脑和肾脏的总CoA池显著高于高碳水化合物或高蛋白饮食的大鼠，而肝脏和骨骼肌的总CoA池显著低于高碳水化合物或高蛋白饮食的大鼠。特别是，肝脏的减少是显著的，这是由Coash水平降低引起的。结果，总CoA池大小减少了喂食其他饮食的大鼠肝脏内容物的大约五分之一。在监测能量平衡的下丘脑中，当大鼠喂食高脂饮食时，测得的所有三种辅酶A分子都处于较高水平。因此，值得注意的是，喂食高脂饮食会影响下丘脑、肝脏和骨骼肌中CoA池的行为，这表明CoA池，特别是丙二酰辅酶A池和/或Coash池与体内的脂质代谢有显著的关系。
Three coenzyme A (CoA) molecular species, i.e., acetyl-CoA, malonyl-CoA, and nonesterified CoA (CoASH), in 13 types of fasted rat tissue were analyzed. A relatively larger pool size of total CoA, consisting of acetyl-CoA, malonyl-CoA, and CoASH, was observed in the medulla oblongata, liver, heart, and brown adipose tissue. Focusing on changes in the CoA pool size in response to the nutrient composition of the diet given, total CoA pools in rats continuously fed a high-fat diet for 4weeks were significantly higher in the hypothalamus, cerebellum, and kidney, and significantly lower in the liver and skeletal muscle than those of rats fed a high-carbohydrate or high-protein diet. In particular, reductions in the liver were remarkable and were caused by decreased CoASH levels. Consequently, the total CoA pool size was reduced by approximately one-fifth of the hepatic contents of rats fed the other diets. In the hypothalamus, which monitors energy balance, all three CoA molecular species measured were at higher levels when rats were fed the high-fat diet. Thus, it was of interest that feeding rats a high-fat diet affected the behaviors of CoA pools in the hypothalamus, liver, and skeletal muscle, suggesting a significant relationship between CoA pools, especially malonyl-CoA and/or CoASH pools, and lipid metabolism in vivo.