Galleria mellonella as a model for fungal pathogenicity testing.
Galleria mellonella as a model for fungal pathogenicity testing.
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DOI：
10.1007/978-1-61779-539-8_33
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Kavanagh, Kevin
中科院分区：
文献类型：
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作者：
Fallon, John;Kelly, Judy;Kavanagh, Kevin
昆虫是用于评估微生物病原体的毒力或用于评估抗微生物药物的有效性的方便模型，并且给出与使用哺乳动物可获得的结果相当的结果。Galleria mellonella幼虫很容易购买和鉴定，并在48小时内提供结果。可以使用各种参数来监测病原体对昆虫的影响，并因此测量其相对毒力。幼虫死亡、免疫细胞（血细胞）数量的变化或病原体在昆虫血腔中的增殖程度是毒力和昆虫免疫反应的良好指标。分析体液免疫反应也可以深入了解病原体与昆虫的相互作用。基因表达的变化或关键抗菌肽的表达提供了昆虫反应的这一要素的数据，并通过外推，哺乳动物免疫系统可能如何反应。G.因此，大蜡螟幼虫提供了一种快速和方便的测定微生物毒力的方法，并且是使用哺乳动物进行这类筛选的有用替代物。
Insects are convenient models for assessing the virulence of microbial pathogens or for assessing the -efficacy of antimicrobial drugs and give results comparable to those that can be obtained using mammals. Galleria mellonella larvae are easy to purchase and inoculate and provide results within 48 h. Various parameters may be used to monitor the effect of a pathogen on the insect and, as a consequence, measure its relative virulence. Larval death, changes in immune cells (haemocytes) numbers, or the extent of proliferation of the pathogen within the insect haemocoel are good indicators of virulence and of the insect's immune response. Analysing the humoral immune response also gives insight into the interaction of the pathogen with the insect. Changes in gene expression or the expression of key antimicrobial peptides provide data on this element of the insect's response and, through extrapolation, how the mammalian immune system might respond. G. mellonella larvae, therefore, provide a quick and convenient means of measuring microbial virulence and are a useful alternative to the use of mammals for this type of screening.