In vivo visualization of nitric oxide and interactions among platelets, leukocytes, and endothelium following hemorrhagic shock and reperfusion
In vivo visualization of nitric oxide and interactions among platelets, leukocytes, and endothelium following hemorrhagic shock and reperfusion
复制标题
DOI：
10.1007/s00011-009-0011-0
复制
发表时间：
2009-08-01
影响因子：
6.7
通讯作者：
Ito, Masaharu
中科院分区：
文献类型：
--
作者：
Hiratsuka, Mie;Katayama, Tomihiro;Ito, Masaharu
采用活体显微镜观察雄性Wistar大鼠失血性休克再灌注（H/R）后0.5和24 h肠系膜微循环中白细胞和血小板的行为，探讨一氧化氮（NO）在H/R后肠系膜微循环中的分布变化及其与白细胞和血小板行为的关系。异硫氰酸荧光素标记的白蛋白的经血管渗漏通过落射照明进行评估。应用4，5-二氨基荧光素二乙酸酯（4，5-diaminofluorescein diacetate，4，5-diaminofluorescein diacetate，4，5-diaminofluorescein diacetate）对NO释放进行显像，H/R组血管白蛋白渗漏及白细胞与血小板粘附均显著增加（P < 0.05）。在H/R 0.5h大鼠，小静脉内皮细胞NO的产生减少。H/R24 h大鼠肥大细胞NO生成量明显增加（P < 0.05）。iNOS抑制可降低白细胞粘附、血小板粘附和微静脉通透性，H/R0.5h后肠系膜内皮细胞功能障碍与NO减少有关，H/R24 h后与肥大细胞NO增加有关。
We examined changes in nitric oxide (NO) distribution in the mesenteric microcirculation after hemorrhagic shock and reperfusion (H/R), and correlated NO production to leukocyte and platelet behavior.The behavior of leukocytes and platelets in mesenteric venules was observed by intravital microscopy at 0.5 and 24 h after H/R in male Wistar rats. Transvascular leakage of fluorescein isothiocyanate-labeled albumin was assessed by epi-illumination. The NO-sensitive dye, 4,5-diaminofluorescein diacetate, was used for imaging NO release.H/R significantly increased vascular albumin leakage and adhesion of leukocytes and platelets (P < 0.05). In H/R 0.5 h rats, NO production in the venular endothelium declined. However, NO production was elevated in H/R 24 h rats in mast cells (P < 0.05). Leukocyte adherence, platelet adherence, and venular permeability were attenuated by iNOS inhibition.Mesenteric endothelial cell dysfunction after H/R 0.5 h is associated with reduced NO, whereas after H/R 24 h is related to increase NO in mast cells.