PRODUCTION OF BACTERIOCINS IN A LIQUID-MEDIUM BY STREPTOCOCCUS-MUTANS
PRODUCTION OF BACTERIOCINS IN A LIQUID-MEDIUM BY STREPTOCOCCUS-MUTANS
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DOI：
10.1128/aac.8.6.707
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发表时间：
1975-01-01
影响因子：
4.9
通讯作者：
DELISLE, AL
中科院分区：
文献类型：
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作者：
DELISLE, AL
由1/2的APT发酵液和4%(wt/vol.)酵母抽提物组成的无菌过滤液体培养基能支持变形链球菌BHT和GS-5产生细菌素。培养上清液调节至pH 7.0，过滤灭菌，含有类细菌素活性，可通过在以化脓性链球菌为敏感指示剂的顶层琼脂草坪上点滴稀释液和向对数相指示剂肉汤培养物中加入稀释液来证明。根据BHT细菌素的致死作用，建立了BHT细菌素的定量检测方法。细菌素的产生直到对数生长期停止后才发生，并且不能被紫外线照射或丝裂霉素C处理诱导。菌株BHT的非细菌素克隆自发产生的频率很高，这表明是由质粒控制的，但这种频率不能通过质粒固化剂丫啶橙和溴化乙锭处理而增加。
A sterile-filtered, liquid medium composed of one-half-strength APT broth and 4% (wt/vol) yeast extract was found to support the production of bacteriocins byStreptococcus mutansstrains BHT and GS-5. Culture supernatants, adjusted to pH 7.0 and sterilized by filtration, contained bacteriocin-like activity, which could be demonstrated by spotting dilutions onto top agar lawns seeded withStreptococcus pyogenesas the sensitive indicator and by adding dilutions to log-phase indicator broth cultures. A quantitative assay was developed for BHT bacteriocin, based on its lethal effects. Bacteriocin production did not occur until after the log phase of growth had ceased and was not inducible by ultraviolet irradiation or treatment with mitomycin C. Non-bacteriocinogenic clones of strain BHT occurred spontaneously at high frequency, suggesting control by a plasmid, but this frequency was not increased by treatment with the plasmid-curing agents acridine orange and ethidium bromide.