Geniposide Prevents PC12 Cells from Peroxynitrite via the Mitogen-Activated Protein Kinase Signaling Pathway
Geniposide Prevents PC12 Cells from Peroxynitrite via the Mitogen-Activated Protein Kinase Signaling Pathway
复制标题
京尼平苷通过丝裂原激活蛋白激酶信号通路阻止 PC12 细胞免受过氧亚硝酸盐的侵害
DOI：
10.1248/jhs.56.195
复制
发表时间：
2010-04-01
影响因子：
--
通讯作者：
Kong, Shuzhen
中科院分区：
文献类型：
--
作者：
Yin, Fei;Liu, Jianhui;Kong, Shuzhen
活性氧在阿尔茨海默病（AD）、帕金森病（PD）和脑卒中等神经退行性疾病的发生发展中起重要作用。本研究探讨京尼平苷对过氧亚硝基阴离子诱导的PC 12细胞的神经保护作用及其信号通路。用Western blotting法检测京尼平苷对PC 12细胞凋亡、Bcl-2、Bcl-2相关X蛋白（Bax）和丝裂原活化蛋白激酶（MAPK）磷酸化的影响。结果表明，京尼平苷可通过上调Bcl-2的表达，对抗SIN-1诱导的PC 12细胞过亚硝酸盐损伤。MAPK/ERK选择性抑制剂U 0126通过抑制MAPK磷酸化和Bcl-2表达而减弱京尼平苷的保护作用。以上结果表明，京尼平苷可抑制MAPK信号通路中SIN-1诱导的PC 12细胞过氧化亚硝酸盐损伤。
Reactive oxygen species play an important role in the development of neurodegenerative diseases, including Alzheimer's disease (AD), Parkinson's disease (PD) and stroke. The present study investigated the neuroprotective action of geniposide and its signaling pathway in peroxynitrite induced PC12 cells. After coculture of PC12 cells with geniposide in the presence or absence of 3-morpholinosydnonmine hydrochloride (SIN-1), a donor of peroxynitrite, Bcl-2, Bcl-2 associated X protein (Bax) and the phosphorylation of mitogen-activated protein kinase (MAPK) were probed with Western blotting. The results showed that geniposide enhanced the expression of Bcl-2 to prevent the peroxynitrite damage induced by SIN-1 in PC12 cells. U0126, a selective inhibitor of MAPK/ERK, attenuated the protective effects of geniposide by inhibiting the phosphorylation of MAPK and expression of Bcl-2. All these data indicate that geniposide prevents PC12 cells from peroxynitrite damage induced by SIN-1 involved in the MAPK signaling pathway.