Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides
Synthesis, structural characterization, and immunological properties of carbon nanotubes functionalized with peptides
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DOI：
10.1021/ja034342r
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发表时间：
2003-05-21
影响因子：
15
通讯作者：
Bianco, A
中科院分区：
文献类型：
--
作者：
Pantarotto, D;Partidos, CD;Bianco, A
碳纳米管（NTs）在药物化学领域的应用具有很高的吸引力。通过开发一种高水溶性碳纳米管的合成方案，解决了在水介质中不溶解的主要问题。因此，生物活性肽可以很容易地通过稳定的共价键连接到碳纳米管。我们已经证明了来自口蹄疫病毒的结合肽，对应于病毒包膜蛋白VP1的141-159区域，保留了结构完整性，并被单克隆和多克隆抗体识别。此外，这种肽- nt偶联物具有免疫原性，可引起正确特异性的抗体反应。这样的系统对诊断目的可能非常有利，并可能在疫苗输送中找到未来的应用。
Carbon nanotubes (NTs) are becoming highly attractive molecules for applications in medicinal chemistry. The main problem of insolubility in aqueous media has been solved by developing a synthetic protocol that allows highly water-soluble carbon NTs to be obtained. As a result, biologically active peptides can be easily linked through a stable covalent bond to carbon NTs. We have demonstrated that a bound peptide from the foot-and-mouth disease virus, corresponding to the 141-159 region of the viral envelope protein VP1, retained the structural integrity and was recognized by monoclonal and polyclonal antibodies. In addition, this peptide-NT conjugate is immunogenic, eliciting antibody responses of the right specificity. Such a system could be greatly advantageous for diagnostic purposes and could find future applications in vaccine delivery.