Blood viscosity during coagulation at different shear rates.
Blood viscosity during coagulation at different shear rates.
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DOI：
10.14814/phy2.12065
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发表时间：
2014-07-01
影响因子：
2.5
通讯作者：
Baryshnikova E
中科院分区：
文献类型：
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作者：
Ranucci M;Laddomada T;Ranucci M;Baryshnikova E
在凝血过程中，血液从液体变为固体凝胶相。这些变化反映在血液粘度的变化，然而，在凝血过程中，在不同的剪切速率（SR）的血液粘度尚未探索。在本研究中，我们使用锥板式粘度计研究了10例凝血功能正常的受试者的全血粘度变化。对于每名受试者，在20、40、80 sec-1的SR下进行三次连续测量。根据血液粘度的时间依赖性变化，我们确定了凝胶点（GP）、凝胶时间点（TGP）、最大凝块粘度（MCV）和凝块溶解半衰期（CLH）。随着SR的增加，TGP显著缩短（P = 0.0023），并且在SR为20 sec−1（P = 0.038）和80 sec−1（P = 0.019）时与活化部分凝血活酶时间显著相关。在SR为80 sec-1时，MCV显著低于SR为40 sec-1时（P = 0.027），随着SR的增加，CLH显著增加（P = 0.048）。这些结果表明，凝血过程中血液粘度的测量提供了许多潜在有用的参数。特别是，TGP和活化部分凝血活酶时间之间的相关性是凝血时间（内在和共同途径）的表达，其因SR增加而缩短可能被解释为SR对血小板活化和凝血酶生成的众所周知的活化作用。需要进一步研究TGP在低凝或高凝状态下的作用，以确认其在临床实践中的作用。使用锥板技术测量血液粘度可以检测凝血过程中血液的液相和凝胶相之间的转变点（凝胶点）。凝胶点时间取决于剪切速率，并与活化部分凝血活酶时间相关。
During the coagulation process, blood changes from a liquid to a solid gel phase. These changes are reflected by changes in blood viscosity; however, blood viscosity at different shear rates (SR) has not been previously explored during the coagulation process. In this study, we investigated the viscosity changes of whole blood in 10 subjects with a normal coagulation profile, using a cone‐on‐plate viscosimeter. For each subject, three consecutive measurements were performed, at a SR of 20, 40, 80 sec−1. On the basis of the time‐dependent changes in blood viscosity, we identified the gel point (GP), the time‐to‐gel point (TGP), the maximum clot viscosity (MCV), and the clot lysis half‐time (CLH). The TGP significantly (P = 0.0023) shortened for increasing SR, and was significantly associated with the activated partial thromboplastin time at a SR of 20 sec−1 (P = 0.038) and 80 sec−1 (P = 0.019). The MCV was significantly lower at a SR of 80 sec−1 versus 40 sec−1 (P = 0.027) and the CLH significantly (P = 0.048) increased for increasing SR. These results demonstrate that measurement of blood viscosity during the coagulation process offers a number of potentially useful parameters. In particular, the association between the TGP and the activated partial thromboplastin time is an expression of the clotting time (intrinsic and common pathway), and its shortening for increasing SR may be interpreted the well‐known activating effects of SR on platelet activation and thrombin generation. Further studies focused on the TGP under conditions of hypo‐ or hypercoagulability are required to confirm its role in the clinical practice. Measurement of blood viscosity with cone‐on‐plate technology allows to detect the transition point between liquid and gel phases of blood during coagulation (gel point). The time‐to‐gel point is dependent on the shear rate and demonstrated an association with the activated partial thromboplastin time.