Green fluorescent protein as a detection marker for Coxiella burnetii transformation
Green fluorescent protein as a detection marker for Coxiella burnetii transformation
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DOI：
10.1016/s0378-1097(99)00203-7
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发表时间：
1999-06-15
影响因子：
2.1
通讯作者：
Barák, I
中科院分区：
文献类型：
--
作者：
Lukácová, M;Valková, D;Barák, I
专性胞内细菌伯氏杆菌的分子生物学研究由于缺乏有效的DNA转化系统而受到阻碍。我们使用绿色荧光蛋白（GFP）和氨苄西林抗性的表达作为检测转化伯氏梭菌细胞的选择标记。荧光显微镜研究表明，转化的伯氏梭菌细胞可以很容易地在宿主细胞系中检测到。在大肠杆菌trc trpllac启动子的作用下，GFP得到了高水平的表达。GFP的使用不仅为伯氏梭菌的转化提供了方便的标记物，而且还可以检测宿主真核细胞内的这种专性细胞内病原体。讨论了绿色荧光蛋白在宿主-病原体相互作用研究中的可能应用。(C) 1999年欧洲微生物学会联合会。Elsevier Science B.V.版权所有。
The molecular biological study of the obligate intracellular bacterium Coxiella burnetii, hampered because of the lack of an efficient DNA transformation system. We used expression of the green fluorescent protein (GFP) in addition to ampicillin resistance as a selection marker for detection of transformed C. burnetii cells. Fluorescent microscopy studies revealed that transformed C. burnetii cells can be detected easily inside the host cell line. A high level of GFP expression was reached with the strong Escherichia coli trc trpllac promoter. The use of GFP not only provides a convenient marker for transformation of C. burnetii, but also allows detection of this obligate intracellular pathogen inside host eukaryotic cells. Possible applications for GFP in the study of host-pathogen interactions are discussed. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.