Markers of malignant transformation of sinonasal inverted papilloma
Markers of malignant transformation of sinonasal inverted papilloma
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DOI：
10.1016/j.ejso.2005.05.014
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发表时间：
2005-10-01
期刊：
影响因子：
3.8
通讯作者：
Tsukuda, M
中科院分区：
文献类型：
--
作者：
Katori, H;Nozawa, A;Tsukuda, M
目的：检测HPV感染状态、表皮生长因子受体（EGFR）和转化生长因子-α（TGF-α）。方法：收集44例鼻窦乳头状瘤和浸润性鳞状细胞癌患者，采用免疫组织化学方法检测TGF-α表达和Ki-67指数。鼻组织用EGFR、TGF-α和Ki-67的单克隆抗体染色。结果进行了分析，采用定量免疫组织化学。分析.结果：EGFR和TGF-α在伴有严重增生异常的IP、伴有癌的IP和浸润性SCC中的表达均显著高于伴有轻度增生异常的IP和对照组。粘膜IP伴不典型增生组Ki-67指数明显升高。在IP中，42%的肿瘤中存在HPV 6/11阳性，31%的肿瘤中存在HPV 16/18阳性。结论：IP癌前病变中EGFR和TGF-α水平明显升高，EGFR和Ki-67的表达与HPV 6/11和16/18的表达密切相关。这些表达可能与IP癌变的早期事件有关。HPV感染可能是IP恶性形成多步骤过程中的早期事件。(c)2005爱思唯尔有限公司保留所有权利。
Aim: To measure HPV status, epidermal growth factor receptor (EGFR) and transforming growth factor-alpha. (TGF-alpha) expression and Ki-67 index in exophytic papilloma (EP), inverted papilloma (IP) with dysplasia, IP with carcinoma and invasive squamous cell carcinoma (SCC).Methods: Forty-four patients with sinonasal papilloma and invasive SCC were selected. The nasal tissues were stained with monoclonal antibodies to EGFR, TGF-alpha and Ki-67. The results were analysed using quantitative immunohistochemical. analysis. In situ hybridization studies for HPV DNA for 6/11, 16/18 and 31/33 were also performed on the tissue.Results: Significant increase of EGFR and TGF-alpha was observed in IP with severe dysptasia, IP with carcinoma and invasive SCC compared to IP with mild dysplasia and control nasal. mucosa. And a serial upreguration in terms of Ki-67 index in IP with dysplasia was observed. Among IP, HPV 6/11-positive was present in 42% tumour and HPV 16/18-positive was present in 31% of tumours. Among HPV 6/11 and 16/18-positive IP, significant increase of EGFR and Ki-67 index were observed.Conclusion: Pre-cancerous Lesions of IP exhibited elevated levels of EGFR and TGF-alpha. and these expression may be associated with early events in IP carcinogenesis. HPV infection may be an early event in a multistep process of malignant formation of IP. (c) 2005 Elsevier Ltd. All rights reserved.