B cell stimulatory factor-1 (interleukin 4) prepares resting murine B cells to secrete IgG1 upon subsequent stimulation with bacterial lipopolysaccharide.
B cell stimulatory factor-1 (interleukin 4) prepares resting murine B cells to secrete IgG1 upon subsequent stimulation with bacterial lipopolysaccharide.
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DOI：
10.4049/jimmunol.139.1.10
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发表时间：
1987-07
影响因子：
4.4
通讯作者：
C. Snapper;W. Paul
中科院分区：
文献类型：
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作者：
C. Snapper;W. Paul
B细胞刺激因子-1（BSF-1）/白细胞介素4显著增强细菌脂多糖（LPS）刺激的B细胞分泌IgG 1和IgE。我们表明，预孵育的休息B细胞与BSF-1单独准备他们分泌IgG 1，但不是IgE，在随后的刺激LPS。BSF-1预孵育在随后加入LPS时增加总活细胞产率的能力仅部分解释了这种增强。增强的程度取决于预孵育的持续时间，最多至少48小时。BSF-1对静息B细胞发挥这种作用，所述静息B细胞已被选择为不存在表面IgG和存在可逆的DNA合成抑制剂羟基脲。在加入LPS之前、同时或之后加入48小时，BSF-1可显著增强IgG 1应答。这些结果强烈地表明，BSF-1在准备分泌IgG 1中的作用模式与LPS无关。
B cell stimulatory factor-1 (BSF-1)/interleukin 4 markedly enhances IgG1 and IgE secretion by B cells stimulated with bacterial lipopolysaccharide (LPS). We show that preincubation of resting B cells with BSF-1 alone prepares them to secrete IgG1, but not IgE, on subsequent stimulation with LPS. The ability of BSF-1 preincubation to increase overall viable cell yield on the subsequent addition of LPS only partially accounts for this enhancement. The degree of enhancement is dependent on the duration of preincubation, up to at least 48 hr. BSF-1 exerts this effect on resting B cells which have been selected for absence of surface IgG and in the presence of the reversible DNA synthesis inhibitor, hydroxyurea. BSF-1 can act to significantly enhance the IgG1 response when added for 48-hr periods before, at the same time as, or after the addition of LPS. These results suggest strongly that the mode of action of BSF-1 in preparing for the secretion of IgG1 is independent of that of LPS.