Analytical Tools To Distinguish the Effects of Localization Error, Confinement, and Medium Elasticity on the Velocity Autocorrelation Function
Analytical Tools To Distinguish the Effects of Localization Error, Confinement, and Medium Elasticity on the Velocity Autocorrelation Function
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DOI：
10.1016/j.bpj.2012.03.062
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发表时间：
2012-06-06
影响因子：
3.4
通讯作者：
Theriot, Julie A.
中科院分区：
文献类型：
--
作者：
Weber, Stephanie C.;Thompson, Michael A.;Theriot, Julie A.
单粒子跟踪是研究生物分子动态行为的一种强有力的技术。然而，许多分析工具容易产生可能导致对底层传输过程错误解释的结果。本文探讨了定位误差和约束对速度自相关函数C-upsilon的影响。我们表明，C-upsilon的计算跨越一系列离散化可以区分定位误差，限制和介质弹性的影响。因此，在某些情况下，C-upsilon可以用作诊断工具，以确定异常扩散的潜在机制。最后，我们将我们的分析应用于大肠杆菌染色体位点和rna -蛋白颗粒的实验数据集。
Single particle tracking is a powerful technique for investigating the dynamic behavior of biological molecules. However, many of the analytical tools are prone to generate results that can lead to mistaken interpretations of the underlying transport process. Here, we explore the effects of localization error and confinement on the velocity autocorrelation function, C-upsilon. We show that calculation of C-upsilon across a range of discretizations can distinguish the effects of localization error, confinement, and medium elasticity. Thus, under certain regimes, C-upsilon can be used as a diagnostic tool to identify the underlying mechanism of anomalous diffusion. Finally, we apply our analysis to experimental data sets of chromosomal loci and RNA-protein particles in Escherichia coli.