Expression of tumor necrosis factor-stimulated gene-6 in the rat ovary in response to an ovulatory dose of gonadotropin.
Expression of tumor necrosis factor-stimulated gene-6 in the rat ovary in response to an ovulatory dose of gonadotropin.
复制标题
DOI：
10.1210/endo.141.11.7784
复制
发表时间：
2000-11
期刊：
影响因子：
4.8
通讯作者：
Shinya Yoshioka;S. Ochsner;Darryl L. Russell;T. Ujioka;Shingo Fujii;J. Richards;L. Espey
中科院分区：
文献类型：
--
作者：
Shinya Yoshioka;S. Ochsner;Darryl L. Russell;T. Ujioka;Shingo Fujii;J. Richards;L. Espey
目前的证据支持这样的假设：哺乳动物排卵的生化事件类似于急性炎症反应。这项研究表明，肿瘤坏死因子刺激基因 6 (TSG-6) 在已诱导排卵的卵泡中表达，该基因编码透明质酸结合蛋白超家族的成员，在炎症反应中特异性翻译。未成熟的 Wistar 大鼠皮下注射 10 IU 马 CG； 48 小时后，皮下注射 10 IU 人 CG (hCG) 启动 12 小时排卵过程。在给动物注射 hCG 后 0、2、4、8、12 和 24 小时提取卵巢 RNA。 RNA 提取物用于 RT-PCR 差异显示扩增的互补 DNA (cDNA)，代表受刺激卵巢组织中的基因表达。对差异扩增的 cDNA 之一进行 Northern 分析证实，它是在 hCG 刺激卵巢后 4-8 小时大幅上调的基因的一部分。亚克隆和序列分析表明该 cDNA 与 TSG-6 基因相匹配。原位杂交表明TSG-6信使RNA主要位于卵丘团和大卵泡的窦颗粒细胞中。总之，数据表明，TSG-6 的表达是排卵卵泡中响应与黄体生成素/hCG 受体偶联的营养激素而发生的炎症样变化级联的一个组成部分。
Current evidence supports the hypothesis that the biochemical events of mammalian ovulation are analogous to an acute inflammatory reaction. This study reveals that tumor necrosis factor-stimulated gene-6 (TSG-6), which encodes a member of the superfamily of hyaluronan-binding proteins that is specifically translated in inflammatory reactions, is expressed in ovarian follicles that have been induced to ovulate. Immature Wistar rats were primed with 10 IU equine CG s.c.; and 48 h later, the 12-h ovulatory process was initiated by 10 IU human CG (hCG), s.c.. Ovarian RNA was extracted at 0, 2, 4, 8, 12, and 24 h after the primed animals were injected with hCG. The RNA extracts were used for RT-PCR differential display of amplified complementary DNAs (cDNAs) that represented gene expression in the stimulated ovarian tissue. Northern analysis of one of the differentially amplified cDNAs confirmed that it was part of a gene that was substantially up-regulated at 4-8 h after the ovaries had been stimulated by hCG. Subcloning and sequence analysis revealed that the cDNA matched the gene for TSG-6. In situ hybridization indicated that the TSG-6 messenger RNA was primarily located in the cumulus mass and the antral granulosa cells of large ovarian follicles. In conclusion, the data show that expression of TSG-6 is an integral part of the cascade of inflammatory-like changes that occur in an ovulatory follicle in response to a trophic hormone that couples with luteinizing hormone/hCG receptors.