Periodontitis increases rheumatic factor serum levels and citrullinated proteins in gingival tissues and alter cytokine balance in arthritic rats.
Periodontitis increases rheumatic factor serum levels and citrullinated proteins in gingival tissues and alter cytokine balance in arthritic rats.
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DOI：
10.1371/journal.pone.0174442
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Casati MZ
中科院分区：
文献类型：
--
作者：
Corrêa MG;Sacchetti SB;Ribeiro FV;Pimentel SP;Casarin RC;Cirano FR;Casati MZ
本研究探讨了实验性牙周炎（EP）和类风湿性关节炎（RA）疾病在关节炎大鼠破坏过程中相互作用的免疫学特征。将大鼠分配至以下组：EP +RA; RA; EP;和阴性对照。通过用II型胶原免疫和用完全弗氏佐剂在爪中局部免疫来诱导RA。结扎右侧第一磨牙诱发牙周炎。采用ELISA法检测EP诱导前（T1）和诱导后28天（T2）血清类风湿因子（RF）和抗瓜氨酸蛋白抗体（ACCPA）水平。ACCPA水平也在T2牙龈组织中进行了测量。对标本进行骨丢失的形态学分析，并收集第一磨牙周围的牙龈组织，使用Luminex/MAGPix测定法定量白细胞介素IL-1β、IL-4、IL-6、IL-17和TNF-α。使用体积描记器分析爪水肿。牙周炎增加RF和ACCPA水平在血清和牙龈组织中，分别。此外，当EP与RA相关联时，EP增加足肿胀的水平并且保持进行直到实验结束。尽管有PE，但仅在RA存在时观察到IL-17的较高值。结论：牙周炎可增加关节炎大鼠血清风湿因子水平和牙龈组织瓜氨酸化蛋白水平，改变细胞因子平衡;同时关节炎可增加牙周组织破坏，证实疾病间存在双向相互作用。
This study investigated some immunological features by experimental periodontitis (EP) and rheumatoid arthritis (RA) disease interact in destructive processes in arthritic rats. Rats were assigned to the following groups: EP +RA; RA; EP; and Negative Control. RA was induced by immunizations with type-II collagen and a local immunization with Complete Freund’s adjuvant in the paw. Periodontitis was induced by ligating the right first molars. The serum level of rheumatoid factor (RF) and anti-citrullinated protein antibody (ACCPA) were measured before the induction of EP (T1) and at 28 days after (T2) by ELISA assay. ACCPA levels were also measured in the gingival tissue at T2. The specimens were processed for morphometric analysis of bone loss, and the gingival tissue surrounding the first molar was collected for the quantification of interleukin IL-1β, IL-4, IL-6, IL-17 and TNF-α using a Luminex/MAGpix assay. Paw edema was analyzed using a plethysmometer. Periodontitis increased the RF and ACCPA levels in the serum and in the gingival tissue, respectively. Besides, the level of paw swelling was increased by EP and remained in progress until the end of the experiment, when EP was associated with RA. Greater values of IL-17 were observed only when RA was present, in spite of PE. It can be concluded that periodontitis increases rheumatic factor serum levels and citrullinated proteins level in gingival tissues and alter cytokine balance in arthritic rats; at the same time, arthritis increases periodontal destruction, confirming the bidirectional interaction between diseases.