Tandem LC columns for the simultaneous retention of polar and nonpolar molecules in comprehensive metabolomics analysis
Tandem LC columns for the simultaneous retention of polar and nonpolar molecules in comprehensive metabolomics analysis
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DOI：
10.1002/jssc.201300779
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发表时间：
2013-11-01
影响因子：
3.1
通讯作者：
McCarry, Brian E.
中科院分区：
文献类型：
--
作者：
Chalcraft, Kenneth R.;McCarry, Brian E.
串联使用亲水相互作用LC柱与RP柱串联配置，导致在单一分析中保留复杂生物样品（小鼠血清）中的极性和非极性组分。该方法成功地将具有正交分离特性的各种柱偶联，采用与两个柱兼容的单一溶剂梯度程序，并使用与TOF质谱仪偶联的ESI进行检测。对于表观容量因子>0.7的洗脱组分，离子抑制（ESI中的一个常见问题）几乎被消除。在三天内进行超过100次进样的串联色谱柱的保留时间重现性与单独使用单柱观察到的保留时间重现性相当。该方法被应用于合并的小鼠血清样品的分析，并提供了高达3000质谱特征的高度可重复的数据。这种方法与传统的LC-MS系统实施，并应找到广泛的适用性，在复杂的混合物，含有广泛的化合物极性的综合分析。
The tandem use of hydrophilic interaction LC columns with RP columns in series configuration has resulted in the retention of both polar and nonpolar components in complex biological samples (mouse serum) in a single analysis. This approach successfully coupled various columns with orthogonal separation characteristics, employed a single solvent gradient program compatible with the two columns and used ESI coupled to a TOF mass spectrometer for detection. Ion suppression, a common problem in ESI, was virtually eliminated for components eluting with apparent capacity factors >0.7. Retention time reproducibility with the tandem columns performed over three days with over 100 injections was comparable to that observed for single columns alone. This method was applied to the analysis of a pooled mouse serum sample and afforded highly reproducible data for up to 3000 mass spectral features. This approach was implemented with a conventional LC-MS system and should find broad applicability in the comprehensive analysis of complex mixtures containing a wide range of compound polarities.