Modulation of MDMA-induced behavioral and transcriptional effects by the delta opioid antagonist naltrindole in mice
Modulation of MDMA-induced behavioral and transcriptional effects by the delta opioid antagonist naltrindole in mice
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DOI：
10.1111/j.1369-1600.2009.00156.x
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发表时间：
2009-07-01
影响因子：
3.4
通讯作者：
Marie-Claire, Cynthia
中科院分区：
文献类型：
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作者：
Belkai, Emilie;Scherrmann, Jean-Michel;Marie-Claire, Cynthia
δ阿片系统涉及各种滥用药物的行为影响。然而，只有少数研究关注阿片类药物系统与 3,4-亚甲基二氧基甲基苯丙胺 (MDMA) 的作用之间可能存在的相互作用。为了研究 δ 阿片类药物系统在 MDMA 诱导的小鼠行为中的可能作用，在纳曲吲哚 (NTI)（一种选择性 δ 阿片类药物拮抗剂）存在的情况下研究了运动活动和条件性位置偏好 (CPP)。此外，通过实时定量聚合酶链反应（QPCR）评估了急性和慢性MDMA给药对脑啡肽原（Penk）和阿片黑皮素原（Pome）基因表达的影响。结果表明，急性 MDMA 给药（9 mg/kg：腹腔注射）后，NTI（5 mg/kg，皮下注射）能够完全阻断 MDMA 引起的过度运动。 Penk 基因表达不受急性 MDMA 调节。但观察到 Pome 基因表达下降。 NTI并没有与之对抗。施用拮抗剂可阻止 MDMA 诱导的 CPP 的获得，表明 δ 阿片受体在这种行为中起作用。长期 MDMA 治疗后，仅 Pome 水平受到调节。观察到的增加被 NTI 预处理完全阻止。所有这些结果证实了 delta 阿片系统（受体和肽）与 MDMA 的作用之间的相互作用。
The delta opioid system is involved in the behavioral effects of various drugs of abuse. However, only a few studies have focused on the possible interactions between the opioid system and the effects of 3,4-methylenedioxymethamphetamine (MDMA). In order to examine the possible role of the delta opioid system in MDMA-induced behaviors in mice, locomotor activity and conditioned place preference (CPP) were investigated in the presence of naltrindole (NTI), a selective delta opioid antagonist. Moreover, the consequences of acute and chronic MDMA administration on pro-enkephalin (Penk) and pro-opiomelanocortin (Pome) gene expression were assessed by real-time quantitative polymerase chain reaction (QPCR). The results showed that, after acute MDMA administration (9 mg/kg: i.p.), NTI (5 mg/kg, s.c.) was able to totally block MDMA-induced hyperlocomotion. Penk gene expression was not modulated by acute MDMA. but a decrease of Pome gene expression was observed. which was not antagonized by NTI. Administration of the antagonist prevented the acquisition of MDMA-induced CPP, suggesting an implication of the delta opioid receptors in this behavior. Following chronic MDMA treatment, only the level of Pome was modulated. The observed increase was totally blocked by NTI pre-treatment. All these results confirm the interactions between the delta opioid system (receptors and peptides) and the effects of MDMA.