Quantum dots encapsulated glycopolymer vesicles: synthesis, lectin recognition and photoluminescent properties.
Quantum dots encapsulated glycopolymer vesicles: synthesis, lectin recognition and photoluminescent properties.
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DOI：
10.1016/j.colsurfb.2015.01.032
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
Danfeng Pei;Yan-Chun Li;Qingrong Huang;Qu Ren;Fan Li;T. Shi
中科院分区：
文献类型：
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作者：
Danfeng Pei;Yan-Chun Li;Qingrong Huang;Qu Ren;Fan Li;T. Shi
采用开环聚合（ROP）和可逆加成-断裂链转移（RAFT）相结合的方法合成了仿生星形聚己内酯-b-聚甲基丙烯酸2-氨基乙酯-b-聚甲基丙烯酸2-氨基乙酯（SPCL-PAMA-PGAMA）共聚物。该糖共聚物自组装成囊泡，具有低临界聚集浓度（CAC）（0.0075 mg/mL）。然后，将羧基封端的CdTe量子点封装在糖基共聚物囊泡内。量子点包裹的糖聚合物囊泡（Gly@QDs囊泡）可以特异性结合刀豆球蛋白A（ConA），而不改变Gly@QDs囊泡的光致发光性质。细胞活力研究表明，Gly@QDs囊泡的细胞毒性较之原来的QDs有了显著的提高。Gly@QDs囊泡被Hep G2细胞内化，然后在细胞中发射绿色荧光。因此，这些Gly@ QD囊泡为靶向递送和成像提供了多功能平台。
Biomimetic star-shaped glycopolymer poly(ɛ-caprolactone)-b-poly(2-aminoethyl methacrylate-b-poly(gluconamidoethylmethacrylate) (SPCL–PAMA–PGAMA) was synthesized by the combination of ring opening polymerization (ROP) and reversible addition-fragmentation chain transfer (RAFT) polymerization. The glycopolymer self-assembled into vesicles with low critical aggregation concentration (CAC) (0.0075 mg/mL). Then, the carboxylic capped CdTe QDs were encapsulated within the glycopolymer vesicles. The QDs encapsulated glycopolymer vesicles (Gly@QDs vesicles) could specifically bind Concanavalin A (Con A) without changing the photoluminescent properties of the Gly@QDs vesicles. Cell viability studies revealed that the cytotoxicity of the Gly@QDs vesicles was remarkably improved as compared to that of the original QDs. The Gly@QDs vesicles were internalized by Hep G2 cells and then emitted green fluorescence in the cells. Consequently, these Gly@QDs vesicles provided a multifunctional platform for targeted delivery and imaging.