What defines a pluripotent hematopoietic stem cell (PHSC): will the real PHSC please stand up!
What defines a pluripotent hematopoietic stem cell (PHSC): will the real PHSC please stand up!
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多能造血干细胞（PHSC）的定义是什么：真正的PHSC请站起来！
作者：
D. Orlic;D. Bodine
在20世纪70年代，Lajtha '。和McC，以及许多评论家定义了多能造血干细胞（PHSCs）的特性。Lajtha和McCulloch主要基于他们自己的观察，将造血干细胞描述为具有广泛增殖潜能和分化为所有造血谱系的能力的细胞。自这一定义提出以来，(1)大量体外和体内造血试验的发展，(2)造血生长因子的鉴定和表征，以及(3)丰富造血细胞亚群的策略的发展，极大地扩展了我们对造血的认识和理解。然而，这些大量的信息并没有改变Lajtha和McCulloch所定义的长期再生造血干细胞的原始概念。在这篇社论中，我们将把我们的评论限制在一些涉及phsc关键特征的论文上。我们不打算对这一领域的所有文献进行全面的回顾。自我更新和长期修复的PHSC特性在小鼠和人类移植受体中被分析得最多。在小鼠实验中，研究人员用有限稀释的高度富集的原始造血细胞或逆转录病毒标记的原始造血细胞，在辐射消融的受体中移植了PHSCs。2628另外，ww小鼠模型已被用作骨髓（BM）移植的非辐照受体。Gardner等人观察到，在辐射消融和微波接收体中重构是等效的。从组织相容的供体中移植具有遗传可区分的染色体、同工酶和血红蛋白（Hb）标记的小鼠骨髓细胞，表明其后代在重组受体的淋巴系、髓系和红系中都有代表。这种方法的一个例子是使用携带T6易位标记的供体细胞。移植4个月后，受体淋巴细胞和髓细胞的有丝分裂图分析显示，几乎每个细胞都有T6标记。M同样，当小鼠移植含有不同葡萄糖-磷酸异构酶3 '和Hb等位基因的BM细胞时？来自供体小鼠的标记基因占分离淋巴细胞、髓细胞和红细胞或红细胞室Hb中葡萄糖-磷酸异构酶的95%以上。逆转录病毒标记显示PHSC可再生
I N THE 1970s, Lajtha’.’ and McC~lloch,~ and many reviewers$” defined the properties of pluripotent hematopoietic stem cells (PHSCs). Based largely on their own observation~,”-’~ Lajtha and McCulloch described PHSCs as cells with extensive proliferative potential and a capacity to differentiate into all hematopoietic lineages. In the years since this definition was proposed, (1) the development of numerous in vitro and in vivo assays for hematopoiesis, (2) the identification and characterization of hematopoietic growth factors, and (3) the development of strategies for enriching subpopulations of hematopoietic cells have greatly expanded our knowledge and understanding of hematopoiesis. However, this large body of information has not changed the original concept of the long-term repopulating hematopoietic stem cell as defined by Lajtha and McCulloch. In this editorial, we will limit our comments to a number of papers that address key features of PHSCs. We will not attempt a complete review of all the literature in this field. The PHSC properties of self-renewal and long-term repopdation have been analyzed most frequently in mouse and human transplant recipients. In mice, PHSCs have been studied in radiation-ablated recipients transplanted with limiting dilutions of highly enriched primitive hematopoietic cell pop~lations’~~’~ or retrovirally marked primitive hematopoietic cells.2628 Alternatively, the W w mouse model has been used as a nonirradiated recipient of bone marrow (BM) transplants. Gardner et air' have observed that reconstitution is equivalent in radiation-ablated and W w recipients. Transplantation of murine BM cells with genetically distinguishable chromosome, isozyme, andor hemoglobin (Hb) markers from histocompatible donors has shown the presence of cells whose progeny are represented in the lymphoid, myeloid, and erythroid lineages of the reconstituted recipient. One example of this approach used donor cells that carry the T6 translocation marker. Four months after transplantation, and analysis of mitotic figures from recipient lymphoid and myeloid cells showed the T6 marker in nearly every cell.M Similarly, when mice were transplanted with BM cells containing different glucose-phosphate isomerase3’ and Hb alleles?’ the marker gene from the donor mouse accounted for greater than 95% of the glucose-phosphate isomerase in isolated lymphoid, myeloid and erythroid cells or Hb in the erythroid compartment. Retroviral marking of PHSC has shown that repopulation
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人类多谱系、自我更新的淋巴造血干细胞在嵌合羊中的持久性。
影响因子：
20.3
作者：
Srour,EF;Zanjani,ED;Cornetta,K;Traycoff,CM;Flake,AW;Hedrick,M;Brandt,JE;Leemhuis,T;Hoffman,R
通讯作者：
Hoffman,R
原始造血干细胞：通过简单的二项式、相关性和协方差计算，通过竞争性再增殖来直接测定最具生产力的细胞群。
影响因子：
2.6
作者：
Harrison,DE;Jordan,CT;Zhong,RK;Astle,CM
通讯作者：
Astle,CM
影响因子：
10.5
作者：
JORDAN, CT;LEMISCHKA, IR
通讯作者：
LEMISCHKA, IR
脐带血和成人骨髓祖细胞的母细胞集落测定。
影响因子：
20.3
作者：
Leary,AG;Ogawa,M
通讯作者：
Ogawa,M
长期骨髓培养干细胞的红细胞生成再增殖能力。
影响因子：
20.3
作者：
Harrison,DE;Lerner,CP;Spooncer,E
通讯作者：
Spooncer,E