Effect of Liquid Oil on the Distribution and Reactivity of a Hydrophobic Solute in Solid Lipid Nanoparticles
Effect of Liquid Oil on the Distribution and Reactivity of a Hydrophobic Solute in Solid Lipid Nanoparticles
复制标题
液体油对固体脂质纳米颗粒中疏水性溶质的分布和反应性的影响
DOI：
10.1007/s11746-013-2228-x
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
J. Coupland
中科院分区：
文献类型：
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作者：
U. Yucel;R. Elias;J. Coupland
固体脂质纳米颗粒的性能通常通过添加少量液体油来改变。研究了添加的液体脂质（十四烷，C14）对自旋探针4-苯基-2，2，5，5-四甲基-3-咪唑啉-1-氧氮氧自由基（PTMIO）在固体脂质纳米粒（二十烷，C20）中的分布和反应性的影响。在EPR分析之前，将用C14：C20（100%C14、90%C20、99%C20和100%C20）的共混物制备并通过酪蛋白酸钠（1重量%）稳定的乳液在21.5 °C或5 °C下储存5或24小时。在液体C14液滴中，PTMIO在液滴和水相之间分配（分别为70%和30%），与储存条件无关。然而，探针在液滴中的比例随着结晶C20浓度的增加而降低。含C20的液滴中PTMIO的分数按以下顺序降低：室温下5 h>室温下24 h>冷藏温度下24 h，并且在具有较高比例的C20的液滴中PTMIO的分数较低。半结晶液滴中的残留PTMIO具有比液体油滴中的PTMIO更高的极性和更低的迁移率，这表明其在围绕结晶脂质核心的层中，并且通过与表面层的相互作用而部分固定。PTMIO的分布模型与水溶液中抗坏血酸离子还原PTMIO的动力学一致。
The performance of solid lipid nanoparticles is often modified by the addition of small amounts of liquid oil. The effects of added liquid lipid (tetradecane, C14) on the distribution and reactivity of the spin probe 4‐phenyl‐2,2,5,5‐tetramethyl‐3‐imidazoline‐1‐oxyl nitroxide (PTMIO) in solid lipid nanoparticles (eicosane, C20) were investigated as a function of storage time and temperature. Emulsions prepared with blends of C14:C20 (100 % C14, 90 % C20, 99 % C20, and 100 % C20) and stabilized by sodium caseinate (1 wt%) were stored at 21.5 °C or 5 °C for 5 or 24 h prior to EPR analyses. In the liquid C14 droplets the PTMIO partitioned between the droplet and aqueous phases (70 and 30 %, respectively) independent of storage conditions. However, the proportion of probe in droplets decreased with increasing crystalline C20 concentration. The fraction of PTMIO in droplets containing C20 decreased in the following sequence: 5 h at room temperature >24 h at room temperature >24 h at refrigerated temperature and was lower in droplets with a higher proportion of C20. The residual PTMIO in semicrystalline droplets has higher polarity and lower mobility than PTMIO in liquid oil droplets suggesting it is in a layer surrounding the crystalline lipid core, and partly immobilized by interaction with the surface layer. The model of PTMIO distribution was consistent with the kinetics of PTMIO reduction by aqueous ascorbate ions.